Oligonucleotide tags for sorting and identification
Patent 5846719 Issued on December 8, 1998. Estimated Expiration Date: 
June 6, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 6, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMultiplex sequencing Multiplex analysis of DNA Method and probe composition for detecting multiple sequences in a single assay Methods for sorting polynucleotides using oligonucleotide tags Oligonucleotide population preparation Patent #: 5658736 InventorsAssigneeApplicationNo. 659453 filed on 06/06/1996US Classes:435/6, Involving nucleic acid536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.2, Non-coding sequences having no known regulatory function which are adaptors or linkers for vector or gene contruction536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/25.4Separation or purification of polynucleotides or oligonucleotidesExaminersPrimary: Chambers, Jasemine C.Assistant: Priebe, Scott D. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C07H 021/00 C07H 021/04 AbstractThe invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention comprise oligonucleotides selected from a minimally cross-hybridizing set. Preferably, such oligonucleotides each consist of a plurality of subunits 3 to 9 nucleotides in length. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.Other References
Field of SearchInvolving nucleic acidVECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Separation or purification of polynucleotides or oligonucleotides Non-coding sequences having no known regulatory function which are adaptors or linkers for vector or gene contruction Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA |
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