High density array fabrication and readout method for a fiber optic biosensor
Patent 5837196 Issued on November 17, 1998. Estimated Expiration Date: 
January 26, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 26, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References H1398 Fluorescent immunoassay employing optical fiber in capillary tube Apparatus including optical fiber for fluorescence immunoassay Immunoassay apparatus Waveguide-binding sensor for use with assays Fiber optic detector for immuno-testing Method and apparatus for the regulation of complex binding Multiple optical fiber event sensor and method of manufacture Optical biosensor and method of use Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample InventorsAssigneeApplicationNo. 592779 filed on 01/26/1996US Classes:422/55, Structured visual or optical indicator, per se65/409, Having plural adjacent fibers or rods sheathed (i.e., bundle) in tube or enclosure422/68.1, Means for analyzing liquid or solid sample422/82.05, Measuring optical property by using ultraviolet, infrared, or visible light422/82.06, Optode or optrode422/82.07, Fluorescence422/82.08, Fluorescence422/82.09, Absorbance or transmittance435/5, Involving virus or bacteriophage435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)524/716Carbohydrate or derivative, e.g., nitrocellulose, etc.ExaminersPrimary: Zitomer, Stephanie W.Assistant: Fredman, Jeffrey Attorney, Agent or FirmForeign Patent References
International ClassesG01N 021/00G01N 015/06 G01N 021/29 C12Q 001/68 AbstractThe invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.Other References
Field of SearchHaving plural adjacent fibers or rods sheathed (i.e., bundle) in tube or enclosurePrior to assembly Means for analyzing liquid or solid sample Measuring optical property by using ultraviolet, infrared, or visible light Optode or optrode Fluorescence Fluorescence Absorbance or transmittance Carbohydrate or derivative, e.g., nitrocellulose, etc. |
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