High throughput screening method for sequences or genetic alterations in nucleic acids
September 13, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for increasing the sensitivity of nucleic acid hybridization assays Detection of mutations in nucleic acids Assay for nucleic acid sequences in an unpurified sample Methods of diagnosing pre-cancer or cancer states using probes for detecting mutant p53 Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit Species-specific DNA-DNA hybridization probe prepared using chromosome size DNA Separation of polynucleotides using supports having a plurality of electrode-containing cells Methods for mapping genetic mutations Probe composition containing a binding domain and polymer chain and methods of use Method and probe composition for detecting multiple sequences in a single assay Patent #: 5514543 InventorAssigneeApplicationNo. 710134 filed on 09/13/1996US Classes:435/5, Involving virus or bacteriophage435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2Acellular exponential or geometric amplification (e.g., PCR, etc.)ExaminersPrimary: Horlick, Kenneth R.Foreign Patent References
International ClassesC12Q 001/70C12Q 001/68 C12P 019/34 AbstractThe present invention provides methods for identifying genetic alterations in a target sequence present in a nucleic acid sample, comprising immobilizing samples on a support, contacting the samples simultaneously with different purine and pyrimidine containing polymers under conditions of hybridization, separating the hybridized polymers from the samples; and identifying the hybridized polymers to identify the genetic alteration(s). The present invention also provides methods for identifying target sequences present in a nucleic acid sample, comprising immobilizing nucleic acid samples on a support, contacting the samples simultaneously different purine and pyrimidine containing polymers under hybridization conditions, separating the polymers from the complementary target sequence(s), and identifying the hybridized polymers to identify the target sequence(s). Further provided by the present invention are methods for identifying randomly permuted genetic alterations in a target sequence present in a nucleic acid sample, comprising immobilizing nucleic acid samples on a support, contacting the samples simultaneously with different purine and pyrimidine containing polymers under hybridization conditions, detecting hybridization between the polymers and the complementary target sequences, to identify the target sequence(s), separating the hybridized polymers from the complementary target sequences; and identifying randomly permuted genetic alterations present in the target sequence.Other References
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