Expression in non-tumoral human lymphoblastoid lines with an integrative vector
Patent 5789247 Issued on August 4, 1998. Estimated Expiration Date: 
August 4, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 4, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Inventors
ApplicationNo. 321613 filed on 10/12/1994US Classes:435/372.2, B-cell or derivative435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)ExaminersPrimary: Robinson, Douglas W.Assistant: Wai, Thanda Attorney, Agent or FirmInternational ClassesC12N 005/22C12N 015/63 C07H 021/04 C12P 021/06 AbstractA plasmid vector designed for transforming lymphoblastoid cells, immortalized by Epstein Barr virus, for producing heterologous proteins, which comprises the bacterial DNA elements required for its amplification together with the following DNA fragments:the DNA elements which constitute an expression cassette allowing the insertion of a heterologous DNA to be expressed in the human lymphoblastoid cells,the Adenovirus 5 derived DNA sequence encoding the VA I and VA II RNA aimed at optimizing the messenger RNA translation,a gene engineered to be efficient as a selective marker in eukaryotic cells, andDNA segments selected for their property to promote plasmid DNA integration into the cell chromosome DNA.Other References
Field of SearchRecombinant DNA technique included in method of making a protein or polypeptideVECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) B-cell or derivative DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) |
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