Method and reagent for simultaneously assaying one or more ligands in a group of preselected ligands

Patent 5789165 Issued on August 4, 1998. Estimated Expiration Date:

October 30, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Nucleic acid-protein conjugate used in immunoassay
Patent #: 4748111
Issued on: 05/31/1988
Inventor: Dattagupta , et al.

Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies
Patent #: 4778751
Issued on: 10/18/1988
Inventor: El Shami , et al.

Multilayer solid phase immunoassay support and method of use
Patent #: 5120662
Issued on: 06/09/1992
Inventor: Chan, et al.

Process for preparing an improved western blot immunoassay Patent #: 5356772
Issued on: 10/18/1994
Inventor: Chan, et al.

Inventors

Assignee

Nissui Pharmaceutical Co., Ltd.

Application

No. 535248 filed on 10/30/1995

US Classes:

435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/7.94, Sandwich assay435/962, PREVENTION OR REMOVAL OF INTERFERING MATERIALS OR REACTANTS OR OTHER TREATMENT TO ENHANCE RESULTS (E.G., DETERMINING OR PREVENTING NONSPECIFIC BINDING, ETC.)435/971, CAPTURE OF COMPLEX AFTER ANTIGEN-ANTIBODY REACTION435/973, SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE435/975, KIT436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/538, Separation of immune complex from unbound antigen or antibody436/541, Absorbent column, particles or resin strip436/808, Automated or kit436/824IMMUNOLOGICAL SEPARATION TECHNIQUES

Examiners

Primary: Spiegel, Carol A.

Foreign Patent References

60-226900 JP. 11/12/1985
63-36151 JP. 02/12/1988
63-188399 JP. 08/12/1988
4-204379 JP. 07/12/1992
4-232465 JP. 08/12/1992

International Class

C12Q 001/68

Foreign Application Priority Data

1993-05-10 JP

Abstract

The assay reagent and kit of the present invention suppress non-specific binding of a labeled substance onto a solid phase, and can assay one or more species of antibodies or one or more species of antigens by means of a single reagent in a simple manner. The assay method involves reacting immunological ligands in a test sample with the assay reagent which contains a combination of components (A) and (B), thereby forming complexes, which complexes are captured onto the independently and separately present Solid phases (C), to assay the label contained in the complexes. Component (A): one or more species of immunological anti-ligand-nucleotide conjugates, in each of which nucleotides with a specific base sequence, independently selected depending on the species of an immunological ligand, are bound to an immunological anti-ligand having a specific immunological affinity to one of the different species of immunological ligands to be assayed; (B): labeled substances each having a specific affinity to one of the different species of immunological ligands to be assayed; Solid phase (C): solid phase-nucleotide conjugates wherein nucleotides, each having a base sequence complementarily binding to a nucleotide of the above component (A), are immobilized onto a water-insoluble support. Either an antigen or an antibody may serve as the aforementioned immunological ligand and immunological anti-ligand.