Patent 5786464 Issued on July 28, 1998. Estimated Expiration Date: July 28, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
536/23.5, Encodes an animal polypeptide435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/91.5, Acellular preparation of polynucleotide435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)536/23.72Viral protein
The invention features a synthetic gene encoding a protein normally expressed in mammalian cells wherein at least one non-preferred or less preferred codon in the natural gene encoding the mammalian protein has been replaced by a preferred codon encoding the same amino acid.
Other References
Fortkamp, E., et al., DNA, vol. 5, No. 6, "Cloning and expression in Escherichia coli of a synthetic DNA for hirudin, the blood coagulation inhibitor in the leech", pp. 511-517, 1986
Cohen, J., et al., "Expression of the HIV-1 env (gp160) in the yeast Saccharomyces cerevisiae via expression/secretion vectors and partial characterization of the gene product", in Modern Approaches, New Vaccines, pages 1988
Chou, K.-C., et al., AIDS Research and Human Retroviruses, vol. 8, No. 12, "Diagrammatization of codon usage in 339 human immunodeficiency virus proteins and its biological implication", pp. 1967-1976, 1992
Rangwala, S. H., et al., Gene, vol. 122, No. 2, "High-level production of active HIV-1 protease in Escherichia coli", pp. 263-269
Scorer, C. A., et al., Gene, vol. 136, Nos. 1 & 2, "The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Picha pastoris", pp. 111-119, 1993
Holler, T. P., et al., Gene, vol. 136, Nos. 1 & 2, "HIV-1 integrase expressed in Escherichia coli from SyntheticC Gene", PP. 323-328 , 1993
Bosch, M. L., et al., Journal of Virology, vol. 68, No. 11, "Insertion of N-linked glycosylation sites in the variable regions of the human immunodeficiency virus type-1 surface protein through AAT triplet reiteration", pp. 7566-7569, 1994
van Hemert, F. J., et al., Journal of Molecular Evolution, vol. 41, No. 2, "The tendency of lentiviral open reading frames to become A-rich: Constraints imposed by viral genome organization and cellular tRNA availability", pp. 132-140, 1995
Cohen, J., et al., (abstract), in Modern Approaches New Vaccines, "Expression of the HIV-1 env (gp160) in the yeast Saccaromyces cerevisiae via expression/secretion vectors and partial characterization of the gene product", 1988
Cochrane et al., "Identification and Charaterization of Intragenic Sequences Which Repress Human Immunodeficiency Virus Structural Gene Expression", Journal of Virology, 65(10):5305-5314, (1991)
D'Agostino et al., "The Rev Protein of Human Immunodeficiency Virus Type 1 Promotes Polysomal Association and Translation of gag/pol and vpu/env mRNAs", Molucular and Cellular Biology, 12(3):1375-1386, (1992)
Feinberg et al., "HTLV-III Expression and Production Involve Complex Regulation at the Levels of Splicing and Translation of Viral RNA", Cell, 46:807-817, (1986)
Hammarskjold et al., "Regulation of Human Immunodeficiency Virus env Expression by the rev Gene Product"Journal of Virology, 63(5):1959-1966, (1989)
Nakamura et al., "Two types of linkage between codon usage and gene-expression levels", FEBS LETTERS, 289(1):123-125, (1991)
Robinson et al., "Codon usage can affect efficiency of translation of genes in Escherichia coli", Nucleic Acids Research, 12(17):6663-6671, (1984)
Zhang et al., "Low-usage codons in Escherichia coli, yeast, fruit fly and primates", Gene, 105(1):61-72 (1991)
Zhang et al., "Graphic Analysis of Codon Usage Strategy in 1490 Human Proteins", Journal of Protein Chemistry, 12(3):329-335, (1993)
Coulombe and Skup, Gene 46:89-95 (1986)
Kamiya et al., Jpn J. Cancer Res. 80:200-203 (1989)
McCarrey, Nucleic Acids Res. 18:949-955 (1990)
Newgard et al., Proc. Natl. Acad. Sci. USA 83:8132-8136 (1986)
Sharp et al., Nucleic Acids Res. 16:8207-8211 (1988)
Inouye et al., FEBS Letters 341:277-280 (1994)
Hernan et al., Biochemistry 31:861908628 (1992)
Williams et al., Nucleic Acids Research 16:10453-10467 (1988