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Method for creating a leukocyte rich sample from a mixed population of blood cells

Patent 5785869 Issued on July 28, 1998. Estimated Expiration Date: Icon_subject July 8, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

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Inventors

Assignee

Application

No. 676988 filed on 07/08/1996

US Classes:

210/782, Blood210/787, Cyclonic, or centrifugal (e.g., whirling or helical motion or by vortex, etc.)210/806, Plural separating435/2, MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT436/177Liberation or purification of sample or separation of material from a sample (e.g., filtering, centrifuging, etc.)

Examiners

Primary: Kim, John

Attorney, Agent or Firm

International Class

B01D 021/26

Abstract

A method and apparatus for creating a leukocyte rich sample from a mixed population of blood cells. The method comprises the steps of: separating an amount of blood into layers that include a buffy coat layer having a mixed population of blood cells including plasma and red blood cells; providing a chamber having an opening for receiving a fluid; causing the buffy coat layer to enter the chamber; centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer; and separating the leukocyte containing layer from the other layers in the chamber.

Other References

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  • Bruisten, S.M. et al. "Efficiency of White Cell Filtration and a Freeze-Thaw Procedure for Removal of HIV-Infected Cells From Blood" in Transfusion, vol. 30, No. 9, 1990, pp. 833-837
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  • Harlowe et al. "Antibodies: A Lab Manual" Cold Spring Harbor, 1988, pp. 584-589
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  • Sutton, Don W. et al. "Cell Separation in the Buffy Coat"in Biorheology, 25, 1988, pp. 663-67
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