Nuclease protection assays
Patent 5770370 Issued on June 23, 1998. Estimated Expiration Date: 
June 14, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 14, 2016. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDetection of base pair mismatches using RNAase A Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof Methods for the synthesis of phosphonate esters Methods for the synthesis of phosphonate esters Very large scale immobilized polymer synthesis Array of oligonucleotides on a solid substrate Patent #: 5445934 InventorAssigneeApplicationNo. 665104 filed on 06/14/1996US Classes:435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/196, Acting on ester bond (3.1)435/199, Ribonuclease (3.1.4)436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.31, Probes for detection of animal nucleotide sequences536/24.32, Probes for detection of microbial nucleotide sequences536/24.33PrimersExaminersPrimary: Zitomer, Stephanie W.Assistant: Tran, Pablo N. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12P 019/34 C07H 021/02 C07H 021/04 AbstractThe invention provides nuclease protection assay comprising: (A) attaching a nucleic acid probe comprising a first nucleotide sequence to a solid surface area; (B) contacting the nucleic acid probe with a nucleic acid template under conditions that promote hybridization between complementary polynucleotides, forming a probe-template complex if the template includes a segment that is complementary to the probe; (C) contacting the probe-template complex with a nuclease effective to selectively cleave the nucleotide bonds of (1) the first nucleotide sequence when the first nucleotide sequence is single stranded or (2) mismatched regions of the first nucleotide sequence when the first nucleotide sequence is in duplex nucleic acid; and (D) detecting the presence of duplex nucleic acids formed by the probe and template nucleic acids by detecting the presence of the first nucleotide sequence.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) Acting on ester bond (3.1) Ribonuclease (3.1.4) BIOSPECIFIC LIGAND BINDING ASSAY DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Probes for detection of animal nucleotide sequences Probes for detection of microbial nucleotide sequences Primers |
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