Heparinase gene from flavobacterium heparinum
Patent 5714376 Issued on February 3, 1998. Estimated Expiration Date: 
February 3, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 3, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesImmobilization of enzymes Process for producing heparinase Process for neutralizing heparin Process for making oligosaccharides having anti-Xa activity and the resulting oligosaccharides Heparin assay Low molecular weight heparin fragments as inhibitors of complement activation Biocompatible protein or ligand immobilization system Process for the sulfation of glycosaminoglycans, the sulfated glycosaminoglycans and their biological applications Patent #: 5013724 InventorsApplicationNo. 783706 filed on 10/23/1991US Classes:435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/2, MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.8, Signal sequence (e.g., beta-galactosidase, etc.)435/71.2, Procaryotic micro-organism435/232, Lyase (4. )435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)ExaminersPrimary: Prouty, Rebecca E.Attorney, Agent or FirmForeign Patent References
International ClassesC12N 015/60C12N 001/21 C12N 009/88 AbstractThe cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.Other References
Field of SearchEncodes an enzymeMAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT Recombinant DNA technique included in method of making a protein or polypeptide Signal sequence (e.g., beta-galactosidase, etc.) Procaryotic micro-organism Lyase (4. ) Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) |
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