Nucleotide fragment of the 23S ribosomal RNA of mycobacteria, derived probes and primers, reagent and detection method
Patent 5703217 Issued on December 30, 1997. Estimated Expiration Date: March 23, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)536/24.32Probes for detection of microbial nucleotide sequences
Bacteria of the genus Mycobacterium can be detected and/or identified by methods using probes including fragments of a variable region of a 23S ribosomal RNA of a species of the genus Mycobacterium or probes including DNA fragments that are obtained by reverse transcription of the RNA or that form the RNA by transcription. Primers for the reverse transcription of a variable region of a 23S ribosomal RNA sequence of mycobacteria include nucleotide sequences of a 23S RNA of a species of the genus Mycobacterium.
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