Patent 5693507 Issued on December 2, 1997. Estimated Expiration Date: December 2, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/470, Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.2, Encodes an enzyme536/23.6, Encodes a plant polypeptide536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)800/293Involving particle-mediated transfecion (e.g., biolistic, etc.)
Novel chimeric constructions and methods for their use are provided for expression of exogenous genes in a plant chloroplast. Particularly, expression is achieved by the use of a chloroplast or bacterial 5' untranslated region in the expression cassette. The expression cassette may be integrated into the chloroplast genome by the use of chloroplast DNA flanking sequences, or may replicate autonomously if provided with a chloroplast origin of replication. Plants and cells containing the transformed chloroplasts are also provided. The constructs may be used with both monocotyledenous and dicotyledenous chloroplasts.
Other References
DeBlock et al. 1985. EMBO J. 4: 1367-1372
Bradley et al. 1985. EMBO J. 4: 3641-3648
Overbeeke et al. 1984. Plant Mol. Biol. 3:235-241
Overbeeke et al. 1984. pp. 461-468. In: Innovations in Biotechnology, Houwink et al., eds., Elsevier: Amsterdam
Klein et al. 1987. Nature 327: 70-73
"Optiminzation of delivery of foreign DNA into hight-plant chloroplasts" Guang-Ning Ye, et al., in Plant Molecular Biology, vol. 15: 809-819 (1990)
"Stable transformation of plastids in higher plants" by Svab et al., in Proceedings of the Natl. Acad. Sci., vol. 87: 8526-8530 (1990)
"Transient foreign gene expression in chloroplasts of cultured tobacco cells after bolistic delivery of chloroplast vectors" by H. Daniell et al. in the Proceedings of the Natl. Acad. Sci. vol. 87: 88-92 (1990)
"Uptake and expression of bacterial and cyanobacterial genes by isolated"Stable cucumber etioplasts by H. Daniell and B.A. McFadden, in Proceedings of the Natl. Acad. Sci., vol. 84: 6349-6353 (1987)
"Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles" by Stephen A. Johnston, et al., in Science, vol. 240: 1538-1541 (1988)
"Chloroplast Transformation in chlamydomonas with High Velocity Microprojectiles" by John E. Boynton, et al., in Science, vol. 240: 1534-1537 (1988)
"Studies on chlamydomonas Chloroplast Transformation: Foreign DNA Can Be Stably Maintained in the Chromosome" by Alan D. Blowers, et al., in The Plant Cell, vol. 1: 123-132 (1989)
"Interspecific chloroplast recombination in a nicotiana somatic hybrid" by Peter Medgyesy, et al., in Proc. Natl. Acad. Sci. USA, vol. 82: 6960-6964 (1985)
"Localization of Replication Origins in Pea Chloroplast DNA" by R. Meeker et al., in Molecular and Cellular Biology, vol. 8: 1216-1223 (1988)
"Leading organellar proteins along new pathways: the relocation of mitochondrial and chloroplast genes to the nucleus" by Phillip Nagley and Rodney J. Devenish, in TIBS, vol. 14: 31-35 (1989)
"Chloroplast promoters" by Handley-Bowden and Chua, in Trends and Biochemical Sciences, vol. 12: 67-70 (1987)
"Isolated cell organelles and subprotoplasts: their roles and somatic cell genetics" by H. Lorz in Plant Genetic Engineering, Chapter 4: 27-57 (1984)