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Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof

Patent 5689052 Issued on November 18, 1997. Estimated Expiration Date: Icon_subject September 19, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Codon pair utilization Patent #: 5082767
Issued on: 01/21/1992
Inventor: Hatfield, et al.

Inventors

Application

No. 530492 filed on 09/19/1995

US Classes:

800/302, Insect resistant plant which is transgenic or mutant536/23.71Bacillus thuringiensis insect toxin

Examiners

Primary: Benzion, Gary

Attorney, Agent or Firm

Foreign Patent References

  • 0359472 EP 03/12/1990
  • WO 93/07278 EP 04/12/1993
  • 90/100076 WO 09/12/1990

International Classes

A01H 005/00
C12N 015/11
C12N 015/82

Abstract

A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided. Also disclosed are novel structural genes which encode insecticidal proteins of B. t. k. and monocotyledonous (e.g. maize) plants containing such novel structural genes.

Other References

  • Murray et al. (1989) Codon Usage in plant genes. Nucleic Acids Research vol. 17, No. 2, pp. 477-498
  • Spencer et al. (1992) Segregation of transgenes in maize. Plant Molecular Biology vol. 18, pp. 201-210
  • Potrykus, I. (1990) Gene Transfer ti Cereals: An Assessment. Bio/Technology, pp. 535-54
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