Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules Patent #: 5429938
ApplicationNo. 487327 filed on 06/07/1995
US Classes:435/325, ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE435/369, Renal origin or derivative435/377, Method of altering the differentiation state of the cell435/397, Support is a gel surface435/400, Hollow514/2, Peptide containing (e.g., protein, peptones, fibrinogen, etc.) DOAI530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES530/399Hormones, e.g., prolactin, thymosin, growth factors, etc.
ExaminersPrimary: Dodson, Shelley A.
Attorney, Agent or Firm
International ClassesA61K 038/18
AbstractA novel cell seeded hollow fiber bioreactor is described as a potential bioartificial kidney. Renal cells are seeded along a hollow fiber in a perfused bioreactor to reproduce the ultrafiltration function and transport function of the kidney. Maintenance of tissue specific function and ultrastructure suggest that this bioreactor provides an economical device for treating renal failure as well as studying renal tubululogenesis in vitro.