Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism

Patent 5686276 Issued on November 11, 1997. Estimated Expiration Date:

May 12, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Inventors

Assignee

E. I. Du Pont de Nemours and Company

Application

No. 440293 filed on 05/12/1995

US Classes:

435/158, Polyhydric435/252.31, Bacillus (e.g., B. subtilis, B. thuringiensis, etc.)435/252.33Escherichia (e.g., E. coli, etc.)

Examiners

Primary: Lilling, Herbert J.

Foreign Patent References

0 361 082 EP. 04/12/1990
0 373 230 EP 06/12/1990
3734 764 DE. 10/12/1987
91 15590 WO. 10/12/1991
93 25696 WO. 12/12/1993

International Classes

C12P 007/18
C12N 015/00
C12N 009/88
C12N 001/19

Abstract

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing either microorganisms containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions.

Other References

Daniel, R. et al, J. of Bacteriology, 177(8), 2151-2156 (1995)
Tobimatsu, T. et al, J. Biolog. Chem., 270(13), 7142-7148 (1995)
Sobolov, M. et al, J. of Bacteriology, 79, 261-266 (1993)
Talarico, T.L. et al., Applied and Environmental Microbiology, Apr. 1990, pp. 943-948
Chemical Abstracts, vol. 118, No. 7, 15 Feb. 1993, Daniel, Rolf et al., "Growth Temperature-Dependent Activity of Glycerol Dehydratase in Escherichia coli Expressing the Citrobacter Freundii dha Regulon"
Chemical Abstracts, vol. 116, No. 9, 2 Mar. 1992, Tong, I Teh et al., "1,3-Propanediol Production by Escherichia coli Expressing Genes from the Klebsiella pneumoniae dha Regulon"
Chemical Abstracts, vol. 120, No. 11, 14 Mar. 1994, Otto, Karin Elizabeth, "Cloning and Characterization of the Propanediol Dehydratase Genes in Salmonella typhimurium"
Chemical Abstracts, vol. 118, No. 17, 26 Apr. 1993, Boenigk, Rainer et al., "Fermentation of Glycerol to 1,3-Propanediol in Continuous Cultures of Citrobacter freundii