Assay preparation containing capture and detection polynucleotides covalently bound to substrates with a heterobifunctional crosslinking agent
January 31, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesPreactivated plastics surfaces for immobilizing organo-chemical and biologic materials Protein immobilization by adsorption of a hydrophobic amidine protein derivative to a hydrophobic surface Covalent attachment of antibodies and antigens to solid phases using extended length heterobifunctional coupling agents Nucleic acid hybridization assay employing detectable anti-hybrid antibodies Polynucleotide capture assay employing in vitro amplification Method and kit for performing nucleic acid hybridization assays Heterofunctional crosslinking agent for immobilizing reagents on plastic substrates Assays and kits incorporating nucleic acid probes containing improved molecular switch Nucleic acid probe, test kit and diagnostic and purification methods Heterobifunctional crosslinked agents for immobilizing molecules on plastic substrates Patent #: 5436147 InventorsApplicationNo. 381231 filed on 01/31/1995US Classes:536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA435/6, Involving nucleic acid435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/181, Attached to the carrier via a bridging agent436/532, Antigen or antibody attached to a carrier via bridging agent530/816Attached to the carrier via a bridging agentExaminersPrimary: Naff, David M.International ClassesC07H 021/04C12N 011/06 G01N 033/549 C12Q 001/68 AbstractHeterofunctional crosslinking agents are used to produce a device for detecting a target polynucleotide. Synthetic single stranded capture and detector polynucleotides are covalently immobilized to plastic surfaces by novel heterofunctional reagents. The capture and detector polynucleotides are covalently attached through a synthetic region of amine-bearing nucleotides that interact with reactive moieties on the heterofunctional crosslinker. The regions of the capture and detector polynucleotides that can hybridize a diagnostically important target polynucleotide are protected from binding to a solid phase support by intrastrand pairing. This system provides monolayers of immobilized polynucleotides with precise orientation and unimpaired diagnostic coding regions.Field of SearchInvolving nucleic acidHaving a fused ring containing a six-membered ring having two N-atoms in the same ring (e.g., purine based mononucleotides, etc.) Attached to the carrier via a bridging agent CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Attached to the carrier via a bridging agent Antigen or antibody attached to a carrier via bridging agent |
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