Large scale DNA microsequencing device
Patent 5661028 Issued on August 26, 1997. Estimated Expiration Date: 
September 29, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 29, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSilicon semiconductor wafer for analyzing micronic biological samples Fluid introduction into a capillary by electroendosmosis Rectangular capillaries for capillary electrophoresis On-capillary gap junction for fluorescence detection in capillary electrophoresis Capillary electrophoresis injection device and method Apparatus and method for an automated electrophoresis system Parallel sequential reactor Method for sequencing polynucleotides Patent #: 5302509 InventorAssigneeApplicationNo. 536010 filed on 09/29/1995US Classes:435/287.2, Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid204/450, Electrophoresis or electro-osmosis processes and electrolyte compositions therefor when not provided for elsewhere204/456, Gel electrophoresis216/39, FORMING GROOVE OR HOLE IN A SUBSTRATE WHICH IS SUBSEQUENTLY FILLED OR COATED423/324, SILICON OR COMPOUND THEREOF435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Sisson, Bradley L.Attorney, Agent or FirmForeign Patent References
International ClassesC12M 001/00C12N 015/00 B60R 009/00 C07H 021/04 AbstractA microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Removing nucleic acid from intact or disrupted cell ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid Including a coated reagent or sample layer Including multiple internal compartments or baffles Including multiple compartments (e.g., wells, etc.) Including optical measuring or testing means DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Primers Synthesis of polynucleotides or oligonucleotides Gel electrophoresis Electrophoresis or electro-osmosis processes and electrolyte compositions therefor when not provided for elsewhere Including means for separating a constituent; e.g., filter, condenser, extractor, etc. With means simultaneously carrying out conjugated reactions within single reactor ANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICE Means for analyzing liquid or solid sample SILICON OR COMPOUND THEREOF ETCHING OF SEMICONDUCTOR MATERIAL TO PRODUCE AN ARTICLE HAVING A NONELECTRICAL FUNCTION FORMING GROOVE OR HOLE IN A SUBSTRATE WHICH IS SUBSEQUENTLY FILLED OR COATED ETCHING TO PRODUCE POROUS OR PERFORATED ARTICLE Etchant contains solid particle (e.g., abrasive for polishing, etc.) |
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