Apparatus for capillary electrophoresis having an auxiliary electroosmotic pump

Patent 5660703 Issued on August 26, 1997. Estimated Expiration Date:

May 31, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

3649499

Electrophoretic apparatus
Patent #: 4459198
Issued on: 07/10/1984
Inventor: Mizuno , et al.

Field-effect electroosmosis
Patent #: 5092972
Issued on: 03/03/1992
Inventor: Ghowsi

Ion-permeable polymer joint for use in capillary electrophoresis
Patent #: 5169510
Issued on: 12/08/1992
Inventor: Lunte, et al.

Capillary electrophoresis method with polymer tube coating
Patent #: 5181999
Issued on: 01/26/1993
Inventor: Wiktorowicz

Capillary electrophoresis apparatus with improved electroosmotic flow control
Patent #: 5320730
Issued on: 06/14/1994
Inventor: Ewing, et al.

Electrical potential configuration for an electrophoresis system
Patent #: 5322607
Issued on: 06/21/1994
Inventor: Baer, et al.

Electrophoresis with chemically suppressed detection
Patent #: 5358612
Issued on: 10/25/1994
Inventor: Dasgupta, et al.

Capillary electrophoresis apparatus with improved electroosmotic flow control Patent #: 5358618
Issued on: 10/25/1994
Inventor: Ewing, et al.

Inventor

Dasgupta, Purnendu K.

Application

No. 455579 filed on 05/31/1995

US Classes:

204/601, Capillary electrophoresis type204/603, With detailed detection system (e.g., including a light source and a camera, etc.)204/604, With injector417/49, Ionic with gettering417/50Electromagnetic

Examiners

Primary: Phasge, Arun S.

Attorney, Agent or Firm

Meier; Linda Blair

International Classes

G01N 027/26
G01N 027/447

Abstract

Apparatus for capillary electrophoresis having an auxiliary electroosmotic pump. Following a conventional capillary electrophoresis system having a single power supply, a separation capillary, and an optical detector, a conductive membrane connects a second capillary to a second power supply. The second capillary and second power supply act together as an auxiliary electroosmotic pump capable of augmenting or inhibiting the bulk electroosmotic flow in the separation capillary. The apparatus can be used to optimize the stacking profile of the sample and, thus, improve the separation efficiencies for charged solutes.

Other References

Dasgupta et al., "Electroosmosis: A reliable fluid propulsion system for flow injection Analysis" (Jun. 1994) pp. 1792-1798, Abstract printed Apr. 15, 1994
Dasgupta, et al., Auxiliary Electroosmotic Pumping in Capillary Electrophoresis, Anal. Chem., vol. 66, No. 19, pp. 3060-3065 Oct. 1994
Dasgupta, et al., Suppressed Conductometric Capillary Electrophoresis Separation Systems, Anal.Chem., vol. 65, pp. 1003-1011 Apr. 1993
Dasgupta, et al., Electroosmosis: A reliable Fluid Propulsion System for Flow Injection Analysis, Anal. Chem., vol. 66, pp. 1792-1798 Jun. 1994
Jorgenson, et al., Zone Electrophoresis in Open-Tubular Glass Capillaries, Anal. Chem, 53, pp. 1298-1302 Jul. 1981
O'Shea, et al., Capillary electrophoresis with electrical chemical detection employing an on-column Nafion joint, J. of Chrom., 593, 1992, pp. 305-312. (no month)
Kok, Off-Column Detection with Pressure Compensation in Capillary Electrophoresis, Anal. Chem, 65, 1853-1860 Jul. 1993
Jorgenson, et al., Science, Capillary Zone Electrophoresis, vol. 222, pp. 266-272 Oct. 1983
Hayes, et al., Electroosmotic Flow Control and Surface Conductance in Capillary Zone Electrophoresis, American Chem. Soc., 65, pp. 2010-2013 Aug. 1993
Datta, et al., Electrokinetic Dispersion in Capillary Electrophoresis, AIChE, vol. 36, No. 6, vol. 36, pp. 916-926 Jun. 1990
Grushka, Effect of hydrostatic flow on the efficiency in capillay electrophoresis, Journal of Chromatography, 1991, 559, pp. 81-93. (no month