Gene sequence induced in skin by retinoids
Patent 5650279 Issued on July 22, 1997. Estimated Expiration Date: 
January 27, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 27, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.InventorsApplicationNo. 379280 filed on 01/27/1995US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.33PrimersExaminersPrimary: Jones, W. GaryAssistant: Rees, Dianne Attorney, Agent or FirmInternational ClassesC12Q 001/68C12P 019/34 C07H 021/02 C07H 021/04 AbstractOne aspect of the invention relates to a novel human cDNA, called TIG1 (Tazarotene Induced Gene 1). Expression of the corresponding TIG1 mRNA is strongly induced from a low basal level upon treatment of skin raft cultures with the RAR ଲ/γ selective anti-psoriatic synthetic retinoid AGN-190168 (ethyl 6-[2-(4,4) dimethyl-thiochroman-6-yl] ethynyl-nicotinate). The TIG1 mRNA is also up-regulated by AGN-190168 and the acid form AGN-190299 (6-[2-(4,4) dimethyl-thiochroman-6-yl] ethynyl-nicotinic acid) in skin raft cultures prepared from psoriatic fibroblasts and normal keratinocytes. Further, the TIG1 mRNA is similarly up-regulated by AGN-190168 in primary fibroblast and keratinocyte cultures. The low basal expression of the TIG1 mRNA is particularly advantageous when used as an indicator of retinoid action in psoriatic skin culture systems. Assay systems employing this unique TIG1 expression profile are disclosed.Other References
Field of SearchInvolving nucleic acidAcellular exponential or geometric amplification (e.g., PCR, etc.) DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Probes for detection of animal nucleotide sequences Probes for detection of microbial nucleotide sequences Primers |
| ||||||||||||||
