Production, purification, cleavage and use of fusion peptides
Patent 5648244 Issued on July 15, 1997. Estimated Expiration Date: 
July 15, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
July 15, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesE. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins Release of recombinant peptides from polypeptides using V8 endopeptidase Patent #: 5087564 InventorsAssigneeApplicationNo. 127692 filed on 09/27/1993US Classes:435/69.7, Fusion proteins or polypeptides435/71.1, Using a micro-organism to make a protein or polypeptide435/71.2, Procaryotic micro-organism435/233, Isomerase (5. )435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/252.33, Escherichia (e.g., E. coli, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)530/344Separation or purificationExaminersPrimary: Wax, Robert A.Assistant: Prouty, Rebecca E. Attorney, Agent or FirmInternational ClassesC12N 015/09C12N 015/63 C12N 015/70 C12N 015/62 AbstractA method for producing a fusion peptide. A vector is provided with nucleic acid encoding a carrier peptide and at the 3' end of the nucleic acid, a unidirectional restriction endonuclease cleavage site recognized by a restriction endonuclease with the ability to create a non-palindromic 3-base overhang. The vector is cleaved with the restriction endonuclease to produce a cleaved vector. One or more nucleic acids encoding a desired peptide and having at least a 3-base overhang at each end configured and arranged for ligation with the cleaved vector is then ligated to the cleavage site.Other References
Field of SearchFusion proteins or polypeptidesUsing a micro-organism to make a protein or polypeptide Procaryotic micro-organism Isomerase (5. ) VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) Escherichia (e.g., E. coli, etc.) Separation or purification |
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