Process for detecting specific mRNA and DNA in cells
Patent 5643730 Issued on July 1, 1997. Estimated Expiration Date: 
March 14, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 14, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDetection and isolation of endorphin mRNA using a synthetic oligodeoxynucleotide Immobilization of message RNA directly from cells onto filter material Process for amplifying, detecting, and/or-cloning nucleic acid sequences Process for amplifying nucleic acid sequences Process for amplifying, detecting, and/or cloning nucleic acid sequences Method and kit for polynucleotide assay including primer-dependant DNA polymerase Methods of extracting nucleic acids and PCR amplification without using a proteolytic enzyme Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction Patent #: 5334499 InventorsApplicationNo. 403555 filed on 03/14/1995US Classes:435/6, Involving nucleic acid435/5, Involving virus or bacteriophage435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/183, ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.32, Probes for detection of microbial nucleotide sequences536/24.33PrimersExaminersPrimary: Jones, W. GaryAssistant: Rees, Dianne Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12Q 001/70 C12P 019/34 C07H 021/04 AbstractThis invention relates to a process for detecting the presence and measuring the quantity of specific mRNA sequences present in in vivo cells or cells maintained in vitro. The process of this invention is applicable to the screening of procaryotic and eucaryotic organisms including the screening of human beings for the presence of disease states. The process of this invention is also applicable to the in vitro screening of the effect or effects of chemical compounds upon one or several gene products as exhibited by the presence and amount of mRNA resulting from transcription of said gene or genes. The process of this invention is particularly suited for screening of a large number of compounds for the effect or effects of compounds upon gene products. This invention also relates to compounds capable of affecting the presence of specific mRNA sequences in cells. The process of this invention also is applicable to the identification of novel gene constructs in viruses, microorganisms, plants and animals. This invention also relates to a novel process for the isolation of RNA and DNA from cells.Other References
Field of SearchInvolving virus or bacteriophageInvolving nucleic acid CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Primers |
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