Molecular biological diagnostic systems including electrodes
Patent 5632957 Issued on May 27, 1997. Estimated Expiration Date: 
September 9, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 9, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSemi-conductor non-volatile optical memory device Mobile ion film memory Detection of microbial nucleic acids by a one-step sandwich hybridization test Sample-scanning photometer Process and apparatus for electrically desorbing components selectively sorbed on an electrolytically conducting barrier Process and apparatus for electrically desorbing components selectively sorbed on granules Methods and kits for performing nucleic acid hybridization assays Method and means for annealing complementary nucleic acid molecules at an accelerated rate Method and apparatus for performing automated, multi-sequential immunoassays Silicon semiconductor wafer for analyzing micronic biological samples InventorsApplicationNo. 304657 filed on 09/09/1994US Classes:422/68.1, Means for analyzing liquid or solid sample257/E21.43, Recessing gate by adding semiconductor material at source (S) or drain (D) location, e.g., transist or with elevated single crystal S and D (EPO)257/E21.435, Lateral single gate single channel silicon transistor with both lightly doped source and drain extensions and source and drain self-aligned to sides of gate, e.g., LDD MOSFET, DDD MOSFET (EPO)257/E29.267, With nonplanar structure (e.g., gate or source or drain being nonplanar) (EPO)422/50, ANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICE422/52, Chemiluminescent422/55, Structured visual or optical indicator, per se422/56, Having reagent in absorbent or bibulous substrate422/61, Test package or kit422/62, Automatic analytical monitor and control of industrial process422/63, Sample mechanical transport means in or for automated analytical system422/67, With specific analytical program control means422/69, Sorption testing422/81, Automated system with sample fluid pressure transport means422/82.01, Measuring electrical property422/82.02, Resistance or conductivity422/82.03, Ion selective electrode422/82.04, Dissolved gas422/82.05, Measuring optical property by using ultraviolet, infrared, or visible light435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/173.1, TREATMENT OF MICRO-ORGANISMS OR ENZYMES WITH ELECTRICAL OR WAVE ENERGY (E.G., MAGNETISM, SONIC WAVES, ETC.)436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/22.1, N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)ExaminersPrimary: Marschel, Ardin H.Attorney, Agent or FirmForeign Patent References
International ClassesC12M 001/34C12M 001/42 C12Q 001/68 AbstractA system for performing molecular biological diagnosis, analysis and multi-step and multiplex reactions utilizes a self-addressable, self-assembling microelectronic system for actively carrying out controlled reactions in microscopic formats. These reactions include most molecular biological procedures, such as nucleic acid hybridization, antibody/antigen reaction, and clinical diagnostics. Multi-step combinatorial biopolymer synthesis may be performed. A controller interfaces with a user via input/output devices, preferably including a graphical display. Independent electronic control is achieved for the individual microlocations. In the preferred embodiment, the controller interfaces with a power supply and interface, the interface providing selective connection to the microlocations, polarity reversal, and optionally selective potential or current levels to individual electrodes. A system for performing sample preparation, hybridization and detection and data analysis integrates multiple steps within a combined system. Charged materials are transported preferably via free field electrophoresis. DNA complexity reduction is achieved preferably by binding of DNA to a support, followed by cleaving unbound materials, such as by restriction enzymes, followed by transport of the cleaved DNA fragments. Active, programmable matrix devices are formed in a variety of formats, including a square matrix pattern with fanned out electrical connections, an array having electrical connections and optionally optical connections from beneath the specific microlocations. A highly automated DNA diagnostic system results.Other References
Field of SearchANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICEChemiluminescent Structured visual or optical indicator, per se Having reagent in absorbent or bibulous substrate Test package or kit Automatic analytical monitor and control of industrial process Sample mechanical transport means in or for automated analytical system With specific analytical program control means Means for analyzing liquid or solid sample Sorption testing Automated system with sample fluid pressure transport means Measuring electrical property Resistance or conductivity Ion selective electrode Dissolved gas Measuring optical property by using ultraviolet, infrared, or visible light Involving nucleic acid Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay TREATMENT OF MICRO-ORGANISMS OR ENZYMES WITH ELECTRICAL OR WAVE ENERGY (E.G., MAGNETISM, SONIC WAVES, ETC.) PACKAGED DEVICE OR KIT BIOSPECIFIC LIGAND BINDING ASSAY N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.) DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) |
| ||||||||||||||
