Enzyme-capture assay (ECA) for the identification of Escherichia coli in clinical samples
Patent 5612186 Issued on March 18, 1997. Estimated Expiration Date: 
June 22, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 22, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesPlant promoter -glucuronidase gene construct Patent #: 5268463 InventorsAssigneeApplicationNo. 263541 filed on 06/22/1994US Classes:435/7.37, Escherichia coli435/7.4, To identify an enzyme or isoenzyme435/7.92Heterogeneous or solid phase assay system (e.g., ELISA, etc.)ExaminersPrimary: Housel, James C.Assistant: Freed, Rachel Heather Attorney, Agent or FirmInternational ClassG01N 033/569AbstractAn enzyme-capture assay (ECA) for rapid identification of Escherichia coli (E. coli) in clinical samples comprising contacting a clinical sample which is suspected to contain E. coli with an immobilized antibody against ଲ-D-glucuronidase which then catalyzes an enzyme substrate to produce a fluorescent product is disclosed.Other References
Field of SearchInvolving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysateBacteria or actinomycetales Escherichia coli To identify an enzyme or isoenzyme Determining presence or kind of micro-organism; use of selective media Heterogeneous or solid phase assay system (e.g., ELISA, etc.) |
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