Active programmable electronic devices for molecular biological analysis and diagnostics
Patent 5605662 Issued on February 25, 1997. Estimated Expiration Date: 
February 25, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 25, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDetection of microbial nucleic acids by a one-step sandwich hybridization test Methods and kits for performing nucleic acid hybridization assays Optimized capacitive sensor for chemical analysis and measurement Silicon semiconductor wafer for analyzing micronic biological samples Method of manufacturing a plurality of uniform microfabricated sensing devices having an immobilized ligand receptor Imaging immunoassay detection system with background compensation and its use Method and device for moving molecules by the application of a plurality of electrical fields Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof Immobolization of biomolecules by enhanced electrophoretic precipitation Method of sequencing of genomes by hybridization of oligonucleotide probes InventorsApplicationNo. 146504 filed on 11/01/1993US Classes:422/68.1, Means for analyzing liquid or solid sample204/600, Electrophoretic or electro-osmotic apparatus204/601, Capillary electrophoresis type257/414, RESPONSIVE TO NON-ELECTRICAL SIGNAL (E.G., CHEMICAL, STRESS, LIGHT, OR MAGNETIC FIELD SENSORS)257/E21.43, Recessing gate by adding semiconductor material at source (S) or drain (D) location, e.g., transist or with elevated single crystal S and D (EPO)257/E21.435, Lateral single gate single channel silicon transistor with both lightly doped source and drain extensions and source and drain self-aligned to sides of gate, e.g., LDD MOSFET, DDD MOSFET (EPO)257/E29.267, With nonplanar structure (e.g., gate or source or drain being nonplanar) (EPO)422/50, ANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICE422/55, Structured visual or optical indicator, per se422/56, Having reagent in absorbent or bibulous substrate422/57, Having coated reagent422/58, In holder or container having special form422/63, Sample mechanical transport means in or for automated analytical system422/69, Sorption testing422/82.01, Measuring electrical property422/82.02, Resistance or conductivity422/82.05, Measuring optical property by using ultraviolet, infrared, or visible light422/82.06, Optode or optrode422/82.07, Fluorescence422/82.08, Fluorescence422/82.09, Absorbance or transmittance422/129, CHEMICAL REACTOR422/131, Organic polymerization422/138, With heat exchanger for reaction chamber or reactants located therein435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/90, Dinucleotide (e.g., NAD, etc.)435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/91.3, Polynucleotide contains only ribonucleotide monomers435/91.5, Acellular preparation of polynucleotide435/91.51, Involving RNA as a starting material or intermediate435/173.1, TREATMENT OF MICRO-ORGANISMS OR ENZYMES WITH ELECTRICAL OR WAVE ENERGY (E.G., MAGNETISM, SONIC WAVES, ETC.)435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/176, Enzyme or microbial cell is immobilized on or in an inorganic carrier435/177, Enzyme or microbial cell is immobilized on or in an organic carrier435/283.1, APPARATUS435/285.1, Mutation or genetic engineering apparatus435/285.2, With means for applying an electric current or charge (e.g., electrofusion, electroporation, etc.)435/287.1, Including measuring or testing435/287.2, Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid435/287.3, With sample or reagent mechanical transport means435/287.7, Including bibulous or absorbent layer435/287.8, Including multiple, stacked layers435/287.9, Including a coated reagent or sample layer435/288.7, Including optical measuring or testing means435/290.1, Composting apparatus435/292.1, Including means to transmit light into a bioreactor to facilitate photo- bioreaction (e.g., photosynthesis)435/299.1, Including solid extended fluid contact reaction surface435/808, OPTICAL SENSING APPARATUS435/814, ENZYME SEPARATION OR PURIFICATION436/63, BIOLOGICAL CELLULAR MATERIAL TESTED436/164, OPTICAL RESULT436/165, With claimed manipulation of container to effect reaction or use of container of claimed optical structure436/166, Including reagent preparation436/169, With reagent in absorbent or bibulous substrate436/172, With fluorescence or luminescence436/175, Digestion or removing interfering materials436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/524, Carrier is inorganic436/525, Metal or metal coated436/528, Carrier is organic436/531, Carrier is synthetic resin436/532, Antigen or antibody attached to a carrier via bridging agent436/535, Antigen or antibody entrapped within the carrier (e.g., gel, hollow fiber, etc.)436/805, OPTICAL PROPERTY438/49Chemically responsiveExaminersPrimary: Marschel, Ardin H.Attorney, Agent or FirmForeign Patent References
International ClassesC12M 001/40C12Q 001/68 C07H 021/02 C07H 021/04 AbstractA self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micromachining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Other References
Field of SearchANALYZER, STRUCTURED INDICATOR, OR MANIPULATIVE LABORATORY DEVICESample mechanical transport means in or for automated analytical system Structured visual or optical indicator, per se Having reagent in absorbent or bibulous substrate Having coated reagent In holder or container having special form Means for analyzing liquid or solid sample Sorption testing Measuring electrical property Resistance or conductivity Measuring optical property by using ultraviolet, infrared, or visible light Optode or optrode Fluorescence Fluorescence Absorbance or transmittance CHEMICAL REACTOR Organic polymerization With heat exchanger for reaction chamber or reactants located therein Involving nucleic acid Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay Dinucleotide (e.g., NAD, etc.) Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) Involving the making of multiple RNA copies Polynucleotide contains only ribonucleotide monomers Acellular preparation of polynucleotide Involving RNA as a starting material or intermediate TREATMENT OF MICRO-ORGANISMS OR ENZYMES WITH ELECTRICAL OR WAVE ENERGY (E.G., MAGNETISM, SONIC WAVES, ETC.) CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF Enzyme or microbial cell is immobilized on or in an inorganic carrier Enzyme or microbial cell is immobilized on or in an organic carrier APPARATUS Mutation or genetic engineering apparatus With means for applying an electric current or charge (e.g., electrofusion, electroporation, etc.) BIOSPECIFIC LIGAND BINDING ASSAY INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS Carrier is inorganic Metal or metal coated Carrier is organic Carrier is synthetic resin Antigen or antibody attached to a carrier via bridging agent Antigen or antibody entrapped within the carrier (e.g., gel, hollow fiber, etc.) BIOLOGICAL CELLULAR MATERIAL TESTED OPTICAL RESULT With claimed manipulation of container to effect reaction or use of container of claimed optical structure Including reagent preparation With reagent in absorbent or bibulous substrate With fluorescence or luminescence Digestion or removing interfering materials OPTICAL PROPERTY N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.) |
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