Patent 5593963 Issued on January 14, 1997. Estimated Expiration Date: January 14, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
514/12, 25 or more peptide repeating units in known peptide chain structure424/93.21, Eukaryotic cell426/53, Animal feed426/54, Preparation of animal feed426/635, Animal food435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.8, Signal sequence (e.g., beta-galactosidase, etc.)435/196, Acting on ester bond (3.1)435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/419, Plant cell or cell line, per se, contains exogenous or foreign nucleic acid514/44, Polynucleotide (e.g., RNA, DNA, etc.)536/23.74, Fungal protein536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)800/298, Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms)800/306, Brassica800/317.3Tobacco
The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
Other References
B. J. C. Cornelissen et al. Nature, vol. 321 (29 May '86) pp. 531-532
T. Ohtani et al. Plant Mol. Biol., vol. 16 ('91) pp. 117-128
B. Larkins et al. J. Cell Biochem, vol. Suppl. O (9 Part C) p. 264
J. Sambrook et al., Molecular Cloning--A Lab. Manual, CSHL Press, 1989, pp. 11.2-11.19, 11.45-11.49, 11.52-11.61
K. Ehrlich et al. Biochemical & Biophysical Research Communications, vol. 195 #1 ('93) pp. 53-57
R. Freedman Cell, vol. 57, (Jun. 30, 1989) pp. 1069-1072
E. Mullaney Mycologia, vol. 85(1) pp. 71-73 (1993)
Altenbach et al., "Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants" Plant Mol. Biol. (1989) 13(5):513-522
Barker et al., "Cellular localization of soybean storage protein messenger RNA in transformed tobacco seeds" Proc. Natl. Acad. Sci. USA (1988) 85(2):458-462
Bustos et al., "Regulation of beta glucuronidase expression in transgenic tobacco plants by an adenosine thymidine-rich cis-acting sequence found upstream of a french bean beta phaseolin gene" Plant Cell (1989) 1(9):839-854
Casey et al., "The structure of plant storage protein genes" Plant Mol. Biol. Reporter (1987) 5(2):261-281
Christen et al., "Cloning of the phytase gene from germinating soybeans" J. Cell Biochem. (1988) Supplement 12, Part C, p. 190, (abstract No. L402)
Ellis et al., "Tissue-specific expression of a pea legumin gene in seeds of Nicotiana plumbaginifolia" Plant Mol. Biol. (1988) 10(3):203-214
Gibson et al., "Phytase expression during seed germination" J. Cell Biochem. (1988) Supplemental 12, Part C, p. 192 (abstract No. L407)
Higgins et al., "The sequence of a pea vicilin gene and its expression in transgenic tobacco plants" Plant Mol. Biol. (1988) 11(5):683-696
Hoffman et al., "Synthesis and protein body deposition of maize 15-kD zein in transgenic tobacco seeds" EMBO J. (1987) 6(11):3213-3222
Iturriaga et al., "Endoplasmic reticulum targeting and glycosylation of hybrid proteins in transgenic tobacco" Plant Cell (1989) 1:381-390
Krebbers et al., "Production of peptides in plant seeds" Trends in Biotechnol. (1990) 8(1):1-3
Shotwell et al., In: The Biochemistry of Plants, vol. 15, Stumpf, P. K. & Conn, E. E., eds. (Academic Press, San Diego, 1989), chap. 7, pp. 297-345
Sijmons et al., "Production of correctly processed human serum albumin in transgenic plants" Biotechnology (1990) 8(3):217-221
Vandekerckhove et al., "Enkephalins produced in transgenic plants using modified 2S seed storage proteins" Biotechnology (1989) 7:929-932
Voelker et al., "In-vitro mutated phytohemagglutinin genes expressed in tobacco seeds: role of glycans in protein targeting and stability" Plant Cell (1989) 1(1):95-104
Han et al., "Phytate hydrolysis in soybean and cottonseed meals by Aspergillus ficuum phytase" J. Agric. Food Chem. (1988) 36(2):259-262
Han, Y. W., "Removal of phytic acid form soybean and cottonseed meals" J. Agric. Food Chem. (1988) 36(6):1181-1183
Patent Abstracts of Japan vol. 9, No. 17, (C-262) [1740] Jan. 24, 1985
Jordano et al., "A sunflower helianthinin gene upstream sequence ensemble contains an enhancer and sites of nuclear protein interaction" Plant Cell (1989) 1(9):855-866
Hesselman et al., "The effect of ଲ-glucanase on the utilizaton of starch and nitrogen by broiler chickens fed on barley of low-or-high-viscosity" Chem. Abstracts. (1986) 105:574 (abstract No. 132749x)
Broz et al., "Effects of beta-glucanase on the feeding value of broiler diets based on barley or oats" Chem. Abstracts (1986) 105:569 (abstract No. 23478n)
Gopaldas et al., "Studies on a wheat-based amylase-rich food" Food Science Technol. Abstracts (1989) 21(8):123 (abstract 8 M 19)
Colot et al., "Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco" EMBO J. (1987) 6(12):3559-3564
Ullah et al., "Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization" Prep. Biochem. (1987) 17(1):63-91
Jahne et al., "Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley" (Hordeum vulgare L.) Plant Cell Reports (1991) 10:1-6
Gibson et al., "Purification and characterizaton of phytase from cotyledons of germinating soybean seeds" Arch. Biochem. Biophys. (1988) 260(2):503-513
Abersold et al., "Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulase" Proc. Natl. Acad. Sci. USA (1987) 84:6970-6974
Jaye et al., "Isolation of human anti-haemophilic factor IX cDNA clone using a unique 52-Base synthetic oligonucleotide probe derived from the amino acid sequence of bovine factor IX" Nucl. Acids Res. (1983) 11(8):2325-2335
Dorel et al., "Transport of proteins to the plant vacuole is not by bulk flow through the secretory system, and requires positive sorting information" J. Cell Biol. (1989) 108:327-337
Della-Chioppa et al., "Protein trafficking in plant cells" Plant Physiol. (1987) 84:965-968
Hoffman et al., "A modified storage protein is synthesized, processed, and degraded in the seeds of transgenic plants" Plant Mol. Biol. (1988) 11:717-729
Biosys Computer Database Abstract of Yang et al., "Purification and characterization of phytase from rat intestinal mucosa" Biochem. Biophys. Acta (1991) 1075(1):75-82. (Biosys No. 92122246)
Freedman et al., "Protein disulfide isomerase: multiple roles in the modification of nascent secretory proteins" Cell (1989) 57:1069-1072
Perlman et al., "A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides" J. Mol. Biol. (1983) 167:391-409
von Heijne, G., "How signal sequences maintain cleavage specificity" J. Mol. Biol. (1984) 173:243-251
Biosys Computer Database Abstract of Goel et al., "Multiple forms of phytase EC-3.1.3.8 in germinating cotyledons of Cucurbita maxima" Phytochemistry (1979) 18(12):1939-1942. (Biosys No. 70046645)
Biosys Computer Database Abstract of Pospisil et al., "The influence of humic acids on phytase activity isolated from wheat seeds" Biol. Plant (1975) 17(6):468-474. (Biosys No. 61051479)
Biosys Computer Database Abstract of Yoshida et al., "Phytase EC-3.1.3.8 activity associated with isolated aleurone particles of rice grains" Agric. Biol. Chem. (1975) 39(1):289-290. (Biosys No. 60051490)
Biosys Computer Database Abstract of Chan et al., "Distribution and properties of calcium atpase EC-3.6.1.3 phytase EC-3.1.3.8 and alkaline phosphatase EC-3.1.3.1 in isolated enterocytes from normal and vitamin D deficient rats" Gut (1983) 24(10):886-892. (Biosys No. 77085639)
Chrispeels, M. J., "Sorting of proteins in the secretory system" Ann. Rev. Plant Physiol. Plant Mol. Biol. (1991) 42:1-24
John et al., "Reduction in the dietary bulk of soya-fortified bulgur wheat gruels with wheat-based amylase-rich food" Food Science Technol. Abstracts (1989) 21(12):124 (abstract No. 12 M 11
January 14, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
