Method for detection of nucleic acid using total internal reflectance
August 31, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesFluorescent immunoassay employing optical fiber in capillary tube Assay apparatus and methods Apparatus including optical fiber for fluorescence immunoassay Method for the determination of species in solution with an optical wave-guide Apparatus for improving the numerical aperture at the input of a fiber optics device Process for amplifying, detecting, and/or-cloning nucleic acid sequences Fluorescent assays, including immunoassays, with feature of flowing sample Immunoassay apparatus Immunoassay apparatus Dose critical in-vivo detection of anti-cancer drug levels in blood InventorsApplicationNo. 522623 filed on 08/31/1995US Classes:435/6, Involving nucleic acid435/91.2Acellular exponential or geometric amplification (e.g., PCR, etc.)ExaminersPrimary: Arthur, Lisa B.Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12P 019/34 AbstractAn apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection over the course of the amplification reaction. A method and apparatus for detecting target nucleic acid is also provided wherein the presence and concentration of target is determined by total internal reflection and coupling of the target to the TIR element by scissile linkage. An improved immunoassay using total internal reflection and differential temperature cycling is further provided.Other References
Field of SearchInvolving nucleic acidAcellular exponential or geometric amplification (e.g., PCR, etc.) Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate Animal cell Bacteria or actinomycetales |
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