Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
Patent 5556748 Issued on September 17, 1996. Estimated Expiration Date: 
September 17, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 17, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDetection of microbial nucleic acids by a one-step sandwich hybridization test Detection of microbial nucleic acids by a one-step sandwich hybridization test Immuno-agglutination particle suspensions Process for the determination of a specifically bindable substance Solution sandwich hybridization, capture and detection of amplified nucleic acids Patent #: 5374524 InventorAssigneeApplicationNo. 737469 filed on 07/30/1991US Classes:435/6, Involving nucleic acid435/7.5, Involving avidin-biotin binding435/25, Involving oxidoreductase435/27, Involving catalase435/28, Involving peroxidase435/29, Involving viable micro-organism435/34, Determining presence or kind of micro-organism; use of selective media435/35, Using radioactive material536/22.1, N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.31, Probes for detection of animal nucleotide sequences536/24.32, Probes for detection of microbial nucleotide sequences536/24.33PrimersExaminersPrimary: Jones, W. GaryAssistant: Marschel, Ardin H. Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/68AbstractThe invention is directed to a sandwich hybridization assay wherein a nucleic acid capture probe is firstly immobilized on an assay plate via masked receptors on the plate. The capture probe is immobilized by the binding of receptor ligands on the capture probe during this first step. Subsequently, target nucleic acid is hybridized to the immobilized capture probe either before or after the hybridization of an indicator nucleic acid probe onto the target. The target is quantified via detection of the immobilized indicator signal.Other References
Field of SearchInvolving nucleic acidInvolving viable micro-organism Determining presence or kind of micro-organism; use of selective media Using radioactive material Sandwich assay Involving oxidoreductase Involving peroxidase Involving avidin-biotin binding Nitrogen containing DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.) Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) Having reagent in absorbent or bibulous substrate |
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