Method and apparatus for optically screening microscopic cells
December 1, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAutomated single-slide staining device Diagnostic method employing a mixture of normally separable protein-coated particles Novel material for immunological assay of biochemical components and a process for the determination of said components Microcapsules sensitized with antibody and a method for measurement of lymphocyte using the same based on cell-mediated immunity Simultaneous assay for T and B lymphocyte populations and subpopulations Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes Method of staining and identifying cells and compositions thereof Therapeutic and diagnostic methods using soluble T cell surface molecules Method and apparatus for optically screening microscopic cells Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential Patent #: 5348859 InventorsAssigneeApplicationNo. 160986 filed on 12/01/1993US Classes:435/7.21, Animal cell435/7.2, Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate435/7.23, Tumor cell or cancer cell435/7.24Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)ExaminersPrimary: Wax, Robert A.Assistant: Moore, William W. Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/50G01N 033/80 G01N 001/28 G01N 021/00 AbstractAn optical screening method and apparatus for identifying both the morphology and selected characteristics or properties expressed by cells, such as cancer cells. The cells are combined with one or more different sets of microspheres, each set having a reactant bond thereto which will bind to a specific molecule which can exist on one or more types of cancer cells. The cells and microspheres are formed as a smear on a slide, stained with a histological type stain and optically viewed to identify the type of cells to which the different sets of microspheres do or do not bind. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof. A fast cancer cell screening method or panel is provided by adding different sets of microspheres having different reactants bound thereto to different sample portions and optically screening the different sample smears. The microspheres also can be removed from a sample portion with any cells bound thereto and compared to another non-modified sample portion to ascertain if the cancer cells of interest have been removed from the sample portion.Other References
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