Electrochemical denaturation of double-stranded nucleic acid
Patent 5527670 Issued on June 18, 1996. Estimated Expiration Date: 
August 9, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 9, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesElectrochemical methods of assay Patent #: 4945045 InventorAssigneeApplicationNo. 288231 filed on 08/09/1994US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/91.52, Involving a ligase (6.)436/94Saccharide (e.g., DNA, etc.)ExaminersPrimary: Horlick, Kenneth R.Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12P 019/34 Foreign Application Priority Data1990-09-12 GBAbstractA process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process disclosed is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process may also employ a promotor compound such as methyl viologen to speed denaturation. The process may be used in the detection of nucleic acid by hybridizing with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.Other References
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