Three-Dimensional mucosal cell and tissue culture system
Patent 5518915 Issued on May 21, 1996. Estimated Expiration Date: April 6, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
424/422, Implant or insert424/484, Matrices424/572, Tissue, other than lymphoid tissue (e.g., connective, etc.)435/1.1, DIFFERENTIATED TISSUE OR ORGAN OTHER THAN BLOOD, PER SE, OR DIFFERENTIATED TISSUE OR ORGAN MAINTAINING; COMPOSITION THEREFOR435/29, Involving viable micro-organism435/32, Testing for antimicrobial activity of a material435/284.1, Differentiated tissue (e.g., organ) perfusion or preservation apparatus435/371, Epithelial origin or derivative435/373, Method of co-culturing cells435/399Fabric, mat, gauze, or fibrous coating
The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
Freshney, "Culture of Animal Cells, A Manual of Basic Technique" 1983, Alan R. Liss, Inc. N.Y
Lydersen, "Layer Scale Cell Culture Technology" 1987, Hansen Publishers, Munich, Vernon, New York