Synthesis and screening of immobilized oligonucleotide arrays
Patent 5510270 Issued on April 23, 1996. Estimated Expiration Date: 
April 23, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 23, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDiagnostic device incorporating a biochemical ligand Process for the simultaneous synthesis of several oligonucleotides on a solid phase Membranes with bound oligonucleotides and peptides Method of improving the biocompatibility of solid surfaces High resolution patterning on solid substrates Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof Large scale photolithographic solid phase synthesis of an array of polymers Patent #: 5405783 InventorsAssigneeApplicationNo. 954519 filed on 09/30/1992US Classes:436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS422/131, Organic polymerization422/134, Including plural sequential reaction stages435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/6, Involving nucleic acid435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/968, HIGH ENERGY SUBSTRATES (E.G., FLUORESCENT, CHEMILUMINESCENT, RADIOACTIVE, ETC.)435/969, MULTIPLE LAYERING OF REACTANTS435/973, SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE436/527, Glass or silica436/530, Cellulose or derivative436/531, Carrier is synthetic resin436/532, Antigen or antibody attached to a carrier via bridging agent536/25.3, Synthesis of polynucleotides or oligonucleotides536/25.31, Deprotection step536/25.32, Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.)536/25.33, Pentavalent phosphorus compound utilized536/25.34Trivalent phosphorus compound utilizedExaminersPrimary: Spiegel, Carol A.Attorney, Agent or FirmForeign Patent References
International ClassG01N 033/53AbstractA method for synthesizing and screening oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of a substrate comprising immobilized nucleotides on its surface, without irradiation of a second predefined region of the substrate. The irradiation step removes a protecting group from the immobilized nucleotides. The substrate is contacted with a first nucleotide to couple the nucleotide to the immobilized nucleotides in the first predefined region without coupling in the second predefined region. At least a part of the first predefined region and at least a part of the second predefined region are subjected to further irradiation. The substrate is contacted with a second nucleotide, which couples to the immobilized nucleotides in at least part of the first and at least part of the second predefined regions. By repeating these steps, an array of diverse oligonucleotides is formed on the substrate. The diverse oligonucleotides are then screened for specific binding to a receptor, e.g., a polynucleotide.Other References
Field of SearchMEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIPHeterogeneous or solid phase assay system (e.g., ELISA, etc.) Involving nucleic acid MULTIPLE LAYERING OF REACTANTS SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE HIGH ENERGY SUBSTRATES (E.G., FLUORESCENT, CHEMILUMINESCENT, RADIOACTIVE, ETC.) Synthesis of polynucleotides or oligonucleotides Deprotection step Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.) Pentavalent phosphorus compound utilized Trivalent phosphorus compound utilized INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS Glass or silica Cellulose or derivative Carrier is synthetic resin Antigen or antibody attached to a carrier via bridging agent APPARATUS INCLUDED IN PROCESS CLAIM (E.G., PHYSICAL SUPPORT STRUCTURES, ETC.) Multifield plates or multicontainer arrays Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc. Analysis, diagnosis, measuring, or testing product (e.g., specimen preparation, microscope slide smearing) Organic polymerization Including plural sequential reaction stages |
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