Indexing linkers
Patent 5508169 Issued on April 16, 1996. Estimated Expiration Date: 
October 28, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 28, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSix base oligonucleotide linkers and methods for their use Universal restriction endonuclease Patent #: 4935357 InventorsAssigneeApplicationNo. 331152 filed on 10/28/1994US Classes:435/6, Involving nucleic acid435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/810, PACKAGED DEVICE OR KIT536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.33, Primers536/25.3Synthesis of polynucleotides or oligonucleotidesExaminersPrimary: Jones, W. GaryAssistant: Sisson, Bradley L. Attorney, Agent or FirmInternational ClassesC12Q 001/68C12P 019/34 C07H 021/02 C12N 015/00 AbstractSynthetic nucleic acid molecules are provided which are called indexing linkers and which can be selectively linked to unknown, or non-identical cohesive ends of nucleic acid fragments. Such indexing linkers are useful for the selective isolation, identification, amplification, labelling, and modification, of nucleic acid fragments, especially subsets of such fragments released by cleavage using Type IIS restriction endonucleases or restriction endonucleases recognizing interrupted palindromic sequences. Sets of such indexing linker molecules are described which are useful for indexing fragments released by cleavage of a genome. Methods are described for distinguishing such fragments using said indexing linker molecules for effecting detection, or further modification of such fragments; and for amplifying such fragments by the polymerase chain reaction.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) PACKAGED DEVICE OR KIT DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Primers Synthesis of polynucleotides or oligonucleotides |
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