Particle bound binding component immunoassay

Patent 5501949 Issued on March 26, 1996. Estimated Expiration Date:

August 15, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

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Inventor

Marshall, David L.

Assignee

Murex Diagnostics Corporation

Application

No. 290487 filed on 08/15/1994

US Classes:

435/5, Involving virus or bacteriophage422/101, Including means for separating a constituent; e.g., filter, condenser, extractor, etc.435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/7.2, Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate435/7.32, Bacteria or actinomycetales435/7.5, Involving avidin-biotin binding435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/7.93, Competitive assay435/7.94, Sandwich assay435/7.95, Indirect assay435/970, TEST STRIP OR TEST SLIDE435/975, KIT436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/523, Carrier is particulate and the particles are of intentionally different sizes or impregnated differently with the immunochemicals436/529, Polysaccharide, carrier (e.g., dextran, etc.)436/531, Carrier is synthetic resin436/532, Antigen or antibody attached to a carrier via bridging agent436/534, Carrier is water suspendible particles436/538, Separation of immune complex from unbound antigen or antibody436/810, Tube, bottle, or dipstick436/815, TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS436/818, Human chorionic gonadotropin436/824IMMUNOLOGICAL SEPARATION TECHNIQUES

Examiners

Primary: Spiegel, Carol A.

Attorney, Agent or Firm

Jones & Askew

Foreign Patent References

0088636 EP 09/24/1983
0168689 EP 01/24/1986
0183442 EP 06/24/1986
WO88/08534 WO 11/24/1988

International Class

C12Q 001/70

Abstract

An immunoassay method for the detection or quantitation of an analyte suspected of being in a solution comprising: (a) combining said specimen, a first binding component, insoluble particles, and second binding component labelled with a signal generating material in a solid phase retention and separation apparatus having a sufficient pore size such that said particles are trapped within said filter yet permitting rapid passage of fluid therethrough in such a manner that an immunological reaction occurs if analyte is present in said specimen, resulting in the formation of an immunocomplex of insolublized first binding component:analyte:second labelled binding component on or within said filter means; (b) separating bound from unbound material; and (c) determining the presence and/or amount of signal produced which is correlative with the amount of analyte present in the solution.

Other References

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