Method of determining an ordered sequence of subfragments of a nucleic acid fragment by hybridization of oligonucleotide probes
Patent 5492806 Issued on February 20, 1996. Estimated Expiration Date: 
April 12, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 12, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDiagnostic test for hepatitis B virus Hybridization assay and kit therefor Process for amplifying nucleic acid sequences Human papillomavirus 43 nucleic acid hybridization probes and methods for employing the same Patent #: 4849334 InventorsApplicationNo. 045912 filed on 04/12/1993US Classes:435/5, Involving virus or bacteriophage435/6Involving nucleic acidExaminersPrimary: Zitomer, Stephanie W.Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/70C12Q 001/68 Foreign Application Priority Data1987-04-01 YUAbstractThe sequence of a given nucleic acid fragment is read by the hybridization and assembly of positively hybridizing exactly complementary oligonucleotide probes through overlapping subfragments. By simultaneous hybridization of nucleic acid subfragments bound onto a filter, representing single-stranded phage vector with a cloned insert, with about 50,000 to 100,000 groups of probes, the main type of which is (A,T,C,G)(A,T,C,G)N8(A,T,C,G), information for computer determination of a sequence of DNA having the complexity of a mammalian genome are obtained in one step. To obtain a maximally completed sequence, three libraries cloned into the phage vector, M13, are used. The process can be easily and entirely robotized for factory reading of complex genomic fragments or DNA molecules.Other References
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