Spatially-addressable immobilization of anti-ligands on surfaces
Patent 5482867 Issued on January 9, 1996. Estimated Expiration Date: April 23, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS427/2.13, Layer formed contains chemical reagent or chemically reacts with substrate (e.g., cell stain or fix, pH paper, immobilized antigen)430/270.1, Radiation sensitive composition or product or process of making435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/7.5, Involving avidin-biotin binding435/7.93, Competitive assay436/527Glass or silica
Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.
Other References
Takaya et al., 1967, Bull. Chem. Soc. Japan 40:2636-2640 The preparation of cis-3, 4-Ureylenethiophane
Takaya et al., 1968, Chem. Abst. 68:29503f. Thiopenes
Patchornik et al., 21 Oct. 1970, J. Am. Chem. Soc. 92(21):6333-6335 Photosensitive Protecting Groups
Amit et al., 1974, J. Org. Chem. 39(2):192-196. Photosensitive protecting groups of amino sugars and their use in glycoside synthesis. 2-nitrobenzyloxycarbonylamino and 6-nitroveratryloxcarbonylamino derivatives
Guire, 1976, Meth. Enz. 1976:280-288 Photochemical Immobilization of Enzymes and Other Biochemicals
Kohn et al., 1977, J. Org. Chem. 42(6):941-948 Syntheses and Spectral Properties of Substituted Imidazolidones and Imidazolines
Hofmann et al., 24 May 1978, J. Am. Chem. Soc. 100(11):3585-3590 Avidin-Biotin Affinity Coulmns. General Methodds for attaching biotin to Peptides and proteins
Hofmann et al., 1982, Biochem. 21:978-984 Avidin binding of carboxyl-substituted biotin and analogues
Ichimura, 1984, J. Pol. Sci. 22:2817-2828 A convenient photochemical method to immobilize enzymes
Forster et al., 1985, Nuc. Acids Res. 13(3):745-761 Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin
Roffman et al., 14 Apr. 1986, Biochem. Biophys. Res. Comm. 136(1):80-85 Selective labeling of functional groups on membrane proteins or glycoproteins using reactive biotin derivatives and 125 I-Streptavidin
Lacey et al., 1987, Analyt. Biochem. 163:151-158 Photobiotin as a sensitive probe for protein labeling
Wilchek et al., 1988, Analyt. Biochem. 171:1-32 The avidin-biotin complex in bioanalytical applications
Ekins, 1989, J. Pharm. Biomed. Anal. 7(2):155-168 Multi-analyte immunoassay
McCray et al., 1989, Ann. Rev. Biophys. Biophys. Chem. 18:239-270 Properties and uses of photoreactive caged compounds
Milburn et al., 1989, Biochem. 28:49-55 Synthesis, Photochemistry, and Biological Activity of a Caged Photolabile Acetylcholine Receptor Ligand
Dower et al., 1991, Ann. Rep. Med. Chem. 26:271-280 Multianalyte microspot immunoassay. The microanalytical `compact disk` of the future
Fodor et al., 15 Feb. 1991, Science 251:767-773. Light-directed, spatially addressable parallel chemical synthesis
Ekins et al., 1992, Ann. Biol. Clin. 50:337-353 Multianalyte microspot immunoassay. The microanalytical `compact disk` of the future
Sigrist et al., Sep. 1992, Bio/Technology 10:1026-1028 Light-dependent, covalent immobilization of biomolecules on `inhert` surfaces
Ekins et al., 1993, Clin. Chem. 39(2):369-370 Multianalyte Testin
April 23, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
