Reagent and method for the determination of cholesterol
Multilayer analytical element for the detection of hydrogen peroxide
Method of treating neuropsychic disturbances by benzodiazepine derivatives and composition therefor Patent #: 4587245
ApplicationNo. 797011 filed on 11/25/1991
US Classes:435/28, Involving peroxidase435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/25, Involving oxidoreductase435/27Involving catalase
ExaminersPrimary: Wityshyn, Michael G.
Assistant: Leary, Louise N.
Attorney, Agent or Firm
Foreign Patent References
International ClassesC12Q 001/28
Foreign Application Priority Data1990-11-30 JP
AbstractA method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.
Field of SearchInvolving peroxidase
Involving glucose or galactose
MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP
Having reagent in absorbent or bibulous substrate