Electrophoresis in low conductivity buffers
Patent 5464517 Issued on November 7, 1995. Estimated Expiration Date: 
January 30, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 30, 2015. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesPolycationic buffers and method for gel electrophoresis of nucleic acids Patent #: 4936963 InventorsAssigneeApplicationNo. 380425 filed on 01/30/1995US Classes:204/453With injectionExaminersPrimary: Niebling, John F.Assistant: Starsiak, John S. Jr. Attorney, Agent or FirmInternational ClassesG01N 027/26G01N 027/447 AbstractElectrophoresis is performed in buffers which exhibit both substantial buffering capacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples:(1) buffering agents with a small number of charged groups per molecule;(2) carrier ampholytes fractionated to a narrow isoelectric point range;(3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and(4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values.These buffering agents are of particular interest in capillary electrophoresis.Other References
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