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Method for stably transforming plastids of multicellular plants

Patent 5451513 Issued on September 19, 1995. Estimated Expiration Date: Icon_subject August 25, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Assignee

Application

No. 111398 filed on 08/25/1993

US Classes:

800/278, METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/414, Tobacco cell or cell line, per se435/418, Plant cell or cell line, per se, is pest or herbicide resistant or pest lethal435/468, Introduction of a polynucleotide molecule into or rearrangement of a nucleic acid within a plant cell435/469, Introduction via Agrobacterium435/470, Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection800/282, The polynucleotide alters pigment production in the plant800/288, Nonplant protein is expressed from the polynucleotide800/292, Involving electroporation800/293, Involving particle-mediated transfecion (e.g., biolistic, etc.)800/294, Via Agrobacterium800/298, Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms)800/317.3Tobacco

Examiners

Primary: Moody, Patricia R.

Attorney, Agent or Firm

Foreign Patent References

  • 0251654 EP 01/13/1988

International Classes

C12N 015/82
C12N 015/90
C12N 015/65
A01H 005/00

Abstract

This invention provides methods for obtaining a plant cell of a multicellular plant, the plastids of which cell have been stably transformed by a foreign DNA of interest. These methods employ selectable markers some of which are nonlethal, some of which are designed to insert into the plastid genome and some of which are designed to be expressible only from the plastid genome and not from the nuclear genome. This invention also provides cells and multicellular plants the plastids of which have been stably transformed by a foreign DNA of interest.

Other References

  • Boynton et al 1988 Science 240: 1534-1538
  • Blowers et al 1989 (Jan.) Plant Cell 1: 123-132
  • DeBlock et al 1985 EMBO Journal 4 (6): 1367-1372
  • Fox et al 1988 Proc Natl Acad Sci USA 85: 7288-7292
  • Daniell et al 1987 Proc Natl Acad Sci USA 84: 6349-6353
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  • Shinozaki et al 1986 EMBO Journal 5 (9): 2043-2049
  • Moll et al 1990 (April) Mol Gen Genet 221: 245-250
  • Svab et al 1990 (Nov.) Proc Natl Acad Sci USA 87: 8526-8530
  • Vancanneyt, G. et al. (1990) Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol. Gen. Genet. 220:245-250
  • Svab, Z. et al. (1990) Aminoglycoside 3"-adenyltransferase confers resistance to spectinomycin in Nicotania tabacum. Plant Molecular Biology 14:197-205. (Feb)
  • Groning, B. R., et al. (1990), Replicative form DNA of abuliton mosaic virus is present in plastids. Mol. Gen. Genet. 220:485-48
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