Method for the colorimetric determination of an analyte by means of benzyl alcohol dehydrogenase and a chromogenic redox indicator
April 8, 2014. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod and sensor electrode system for the electrochemical determination of an analyte or an oxidoreductase as well as the use of suitable compounds therefor Colorimetric assay by enzymatic oxidation in the presence of an aromatic nitroso or oxime compound 3-aminopyrazolo heterocyclic derivatives, and use for colorimetric determinations Use of a sparingly soluble salt of a heteropoly acid for the determination of an analyte, a corresponding method of determination as a suitable agent therefor Patent #: 5240860 InventorAssigneeApplicationNo. 225722 filed on 04/08/1994US Classes:435/26, Involving dehydrogenase435/25, Involving oxidoreductase436/111, Amine and quaternary ammonium436/112, Tertiary amine436/113, Ammonia546/119, Two of the ring nitrogens are bonded directly to each other546/121Ring nitrogen is shared by the two cyclosExaminersPrimary: Beisner, William H.Assistant: Gitomer, Ralph Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/26Foreign Application Priority Data1993-04-08 DEAbstractThe invention concerns a method for the colorimetric determination of NADH by means of enzymatic oxidation with benzyl alcohol dehydrogenase whereby an aromatic nitrosoaniline compound of the general formula I is reduced to a colored quinone diimine ##STR1## in which R1 denotes hydrogen, halogen, hydroxy, alkoxy, aryloxy, alkylthio, arylthio, alkyl or alkylene substituted if desired by carboxy, PO3 H2, dialkylphosphinyl or SO3 H, amino substituted if desired once or twice by alkyl which in turn can be substituted if desired by hydroxy, PO3 H2, SO3 H or carboxy and R denotes a residue which forms a color by electron conjugation with a quinone diimiine system. The invention also concerns a corresponding agent as well as a chromogenic nitroso compound of formula I as well as its use for the enzymatic colorimetric determination of an analyte.Field of SearchInvolving dehydrogenaseInvolving oxidoreductase Bicyclo ring system having the six-membered hetero ring as one of the cyclos Two of the ring nitrogens are bonded directly to each other Ring nitrogen is shared by the two cyclos Amine and quaternary ammonium Tertiary amine Ammonia TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS |
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