Array of oligonucleotides on a solid substrate
September 30, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDiagnostic device incorporating a biochemical ligand Process for the simultaneous synthesis of several oligonucleotides on a solid phase Self-assembled nanometer lithographic masks and templates and method for parallel fabrication of nanometer scale multi-device structures Use of volume exclusion agents for the enhancement of in situ hybridization In-situ hybridization to detect nucleic acid sequences in morphologically intact cells Membranes with bound oligonucleotides and peptides Method of improving the biocompatibility of solid surfaces High resolution patterning on solid substrates Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof Method of sequencing of genomes by hybridization of oligonucleotide probes Patent #: 5202231 InventorsAssigneeApplicationNo. 954646 filed on 09/30/1992US Classes:435/6, Involving nucleic acid428/426, Of quartz or glass428/532, Of carbohydrate435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/969, MULTIPLE LAYERING OF REACTANTS435/973, SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/527, Glass or silica436/807, APPARATUS INCLUDED IN PROCESS CLAIM (E.G., PHYSICAL SUPPORT STRUCTURES, ETC.)436/809, Multifield plates or multicontainer arrays536/25.3Synthesis of polynucleotides or oligonucleotidesExaminersPrimary: Kepplinger, Esther M.Assistant: Green, Theodore M. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68G01N 033/543 AbstractA method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.Other References
Field of SearchHeterogeneous or solid phase assay system (e.g., ELISA, etc.)Sandwich assay Indirect assay MULTIPLE LAYERING OF REACTANTS SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE Involving nucleic acid Synthesis of polynucleotides or oligonucleotides Deprotection step Labels or markers utilized (e.g., radiotracer, affinity, fluoroescent, phosphorescent, markers, etc.) Pentavalent phosphorus compound utilized Trivalent phosphorus compound utilized INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS Glass or silica APPARATUS INCLUDED IN PROCESS CLAIM (E.G., PHYSICAL SUPPORT STRUCTURES, ETC.) Multifield plates or multicontainer arrays Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc. Next to aldehyde or ketone condensation product Of carbohydrate |
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