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Cloning and expression of phytase from aspergillus

Patent 5436156 Issued on July 25, 1995. Estimated Expiration Date: Icon_subject November 12, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

3297548

Inventors

Assignee

Application

No. 151574 filed on 11/12/1993

US Classes:

435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/196, Acting on ester bond (3.1)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.2Encodes an enzyme

Examiners

Primary: Wax, Robert A.
Assistant: Hendricks, Keith

Attorney, Agent or Firm

Foreign Patent References

  • 0321004 EP. 06/13/1986
  • 0287152 EP. 10/13/1988

International Classes

C12N 015/00
C12N 009/16

Foreign Application Priority Data

1989-09-27 EP

Abstract

A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.

Other References

  • Suggs et al. PNAS vol. 78, No. 11, pp. 6613-6617, Nov. 1981
  • Young et al. PNAS vol. 80, pp. 1194-1198, Mar. 1983
  • Graf, E., ed., Phytic acid: Chemistry and Applications (1986) Pilatus Press, Minneapolis, Minn., Chapter 2, pp. 23-42
  • Powar et al., J. Bacteriol. (1982) 151(3):1102-1108
  • Cosgrove, Austral. J. Biol. Sci. (1970) 23:1207-1220
  • Nayini et al., Lebensmittel Wissenschaft und Technologie (1984) 17:24-26
  • Yamada et al., Agric. Biol. Chem. (1968) 32(10):1275-1282
  • Nelson et al., J. Nutrition (1971) 101:1289-1294
  • Han, Animal Feed Sci. & Technology (1989) 24:345-350
  • Product Information Brochure published by Alko Ltd., entitled "Finaseā„¢ Enzymes by Alko" Rajamaki, Finland, 4 pages total, undated
  • Ullah, Preparative Biochem. (1988) 18(4):443-458
  • Ullah, Enzyme and Engineering Conference IX, Oct. 4-8, 1987, Santa Barbara, Calif. (poster presentation) 2 pages total
  • Ullah, Preparatory Biochem. (1988) 18(4):459-471
  • Ullah, et al., Preparatory Biochem. (1987) 17(1):63-91
  • Mullaney et al., Filamentous Fungi Conference (Apr., 1987) Pacific Grove, Calif. (poster presentation) 15 pages total
  • Samson et al., Nature (1985) 318:191-19
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