Process for specimen handling for analysis of nucleic acids
Patent 5436129 Issued on July 25, 1995. Estimated Expiration Date: 
October 12, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 12, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesProcess for amplifying, detecting, and/or-cloning nucleic acid sequences In situ transcription in cells and tissues Detection of minimal numbers of neoplastic cells carrying DNA translocations by DNA sequence amplification Patent #: 5024934 InventorAssigneeApplicationNo. 135131 filed on 10/12/1993US Classes:435/6, Involving nucleic acid204/450, Electrophoresis or electro-osmosis processes and electrolyte compositions therefor when not provided for elsewhere204/466, Using slab gel422/56, Having reagent in absorbent or bibulous substrate422/58, In holder or container having special form422/61, Test package or kit422/69, Sorption testing422/101, Including means for separating a constituent; e.g., filter, condenser, extractor, etc.422/102, Container422/104, Holder, support, housing, or hood422/947, Using capillary action, including fluid transfer through absorbent matrix [B01L 3/00C6C]435/91.1, Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/287.2, Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid436/177, Liberation or purification of sample or separation of material from a sample (e.g., filtering, centrifuging, etc.)436/178, Including use of a solid sorbent, semipermeable membrane, or liquid extraction436/515, Through a gel (e.g., Ouchterlony technique, etc.)436/516ImmunoelectrophoresisExaminersPrimary: Parr, MargaretAssistant: Sisson, Bradley L. Attorney, Agent or FirmInternational ClassesC12Q 001/68C12P 019/34 C25B 001/00 B01D 061/42 AbstractA process for handling a biological specimen for the analysis of nucleic acid sequences wherein the biological specimen is immobilized within a carrier device for controlled temperature conditions and the sequential addition of fluid treatments. The fluid treatments arm selected from lysing end denaturing solutions, wash or rinse solutions, reagents for nucleic acid amplification, electrophoresis and hybridization and labeling and detection reagents. The fluid treatments are unique to each specimen and the detection of target nucleic acid sequences is localized within the carrier device.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) APPARATUS INCLUDED IN PROCESS CLAIM (E.G., PHYSICAL SUPPORT STRUCTURES, ETC.) HEPATITIS ASSOCIATED ANTIGENS AND ANTIBODIES Liberation or purification of sample or separation of material from a sample (e.g., filtering, centrifuging, etc.) Including use of a solid sorbent, semipermeable membrane, or liquid extraction Through a gel (e.g., Ouchterlony technique, etc.) Immunoelectrophoresis Having reagent in absorbent or bibulous substrate In holder or container having special form Test package or kit Sorption testing Including means for separating a constituent; e.g., filter, condenser, extractor, etc. Container Holder, support, housing, or hood |
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