U.S. patents available from 1976 to present.
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Method for purifying egg yolk immunoglobulins

Patent 5420253 Issued on May 30, 1995. Estimated Expiration Date: Icon_subject September 9, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Manufacture and use of fowl egg antibodies
Patent #: 4550019
Issued on: 10/29/1985
Inventor: Polson

Method of producing high aqueous volume multilamellar vesicles
Patent #: 4855090
Issued on: 08/08/1989
Inventor: Wallach

Steroidal liposomes exhibiting enhanced stability
Patent #: 5100662
Issued on: 03/31/1992
Inventor: Bolcsak, et al.

Modular injection system for avian embryos Patent #: 5136979
Issued on: 08/11/1992
Inventor: Paul, et al.

Inventors

Assignee

Application

No. 118514 filed on 09/09/1993

US Classes:

530/423, Oxygenated material424/157.1, Derived from, or present in, food product (e.g., milk, colostrum, whey, eggs, etc.)530/853, Ovary; eggs; embryos530/861INVOLVING IGG3, IGG4, IGA, OR IGY

Examiners

Primary: Nucker, Christine M.
Assistant: Krsek-Staples, Julie

Attorney, Agent or Firm

International Classes

A23J 001/08
C07K 001/30
C07K 016/02

Abstract

The present invention provides a method for purifying high yields of IgG immunoglobulins from an egg yolk by a single phase separation step using a nonionic detergent.

Other References

  • England et al. "Precipitation Techniques" in Guide to Protein Purification, Deutscher et al ed. Academic Press, Inc. San Diego. (Methods in Enzymology 182: 285-300, 1990)
  • Neugebauer, J. M., "Detergents: An Overview" in Guide to Protein Purification Deutscher et al. Academic Press, Inc. San Diego (Methods in Enzymology 182: 239-253, 1990)
  • Helenius et al, Biochimica et Biophysica Acta 415: 29-79, 1975
  • Hjelmeland L. M., "Solubilization of Membrane Proteins" in Guide to Protein Purification, Deutscher et al ed. Academic Press, San Diego. (Methods in Enzymology 182: 253-264, 1990)
  • "Appendix B: Preparation of Reagents and Buffers Used in Molecular Cloning", B.1
  • Bade et al., "Rapid Method of Extraction of Antibodies from Hen Egg Yolk", J. of Immuno. Methods, 72, 421-426 (1984)
  • Bollag et al., "Polyethylene Glycol (PEG) Precipitation", Protein Methods, Wiley-Liss, 84
  • Bordier, "Phase Separation of Integral Membrane Proteins in Triton X-114 Solution", J. Biol. Chem., 256, 1604-1607 (1981)
  • Cams et al., "Surfactants and Detersive Systems", Kirk Othmer (Concise Encyclopedia of Chemical Technology), 1142-1146 (1985)
  • Goding, Monoclonal Antibodies: Principles and Practice, 2d ed., Academic Press, 161-164 (1986)
  • Goudswaard et al., "The Immunoglobulins of the Turkey (Meleagris gallopavo) Isolation and Characterization of IgG, IgM and IgA in Body Fluids, Eggs and Intraocular Tissues", Poultry Science, 56, 1847-1851 (1977)
  • Hadge et al., "Evolution of Low Molecular Weight Immunoglobulins", Developmental and Comparative Immunology, 4, 501-513 (1980)
  • Harlow et al., "Detergents", Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 687-689 (1988)
  • Hassl et al., "Purification of Egg Yolk Immunoglobulins: A Two-Step Procedures Using Hydrophobic Interaction Chromatography and Gel Filtration", J. of Immuno. Methods, 110, 225-228 (1988)
  • Jensenius et al., "Eggs: Conveniently Packaged Antibodies. Methods for Purification of Yolk IgG", J. of Immuno. Methods, 46, 63-68 (1981)
  • Larsson et al., "Chicken Antibodies: Taking Advantage of Evolution--A Review", Poultry Science, 72, 1807-1812 (1993)
  • Losch et al., "The Chicken Egg, an Antibody Source", J. Vet. Med., 33, 609-619 (1986)
  • Polson et al., "Isolation of Viral IgY Antibodies from Yolks of Immunized Hens", Immuno. Communications, 9, 475-493 (1980)
  • Polson et al., "Antibodies to Proteins from Yolk of Immunized Hens", Immuno. Communications, 9, 495-513 (1980)
  • Polson et al., "Improvements in the Isolation of IgY from the Yolks of Eggs Laid by Immunized Hens", Marcel Dekker, Inc., 323-327 (1985)
  • Rose et al., "Immunoglobulins in the Egg, Embryo and Young Chick", Developmental and Comparative Immunology, 5, 15-20 and 371-375, (1981)
  • Shah et al., "Separating Yolk Proteins and Lipids", Journal of Food Processing and Preservation, 16, 275 (1992)
  • Yokoyama et al., "Passive Protective Effect of Chicken Egg Yolk Immunoglobulins Against Experimental Enterotoxigenic Escherichia coli Infection in Neonatal Piglets", Infection and Immunity, 60, 998-1007 (1992
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