Method for soybean transformation and regeneration

Patent 5416011 Issued on May 16, 1995. Estimated Expiration Date:

November 23, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Process for regenerating soybeans
Patent #: 4684612
Issued on: 08/04/1987
Inventor: Hemphill , et al.

Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
Patent #: 4769061
Issued on: 09/06/1988
Inventor: Comai

Method of regenerating soybeans from cultured soybean cotyledonary nodes
Patent #: 4992375
Issued on: 02/12/1991
Inventor: Wright

Particle-mediated transformation of soybean plants and lines
Patent #: 5015580
Issued on: 05/14/1991
Inventor: Christou, et al.

Method for transforming plants via the shoot apex
Patent #: 5164310
Issued on: 11/17/1992
Inventor: Smith, et al.

Glyphosate-resistant plants Patent #: 5188642
Issued on: 02/23/1993
Inventor: Shah, et al.

Inventors

Application

No. 156611 filed on 11/23/1993

US Classes:

800/294Via Agrobacterium

Examiners

Primary: Chereskin, Che S.

Attorney, Agent or Firm

Lavin, Jr.; Lawrence M., Hoerner, Jr.; Dennis R., Shear; Richard H.

Foreign Patent References

0218571 EP. 04/13/1987
94/02620 WO. 02/13/1994

International Class

C12N 015/82

Abstract

A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

Other References

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