Amplification of midivariant DNA templates
Patent 5407798 Issued on April 18, 1995. Estimated Expiration Date: 
February 5, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
February 5, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAutocatalytic replication of recombinant RNA Detection of specific sequences in nucleic acids Target-dependent synthesis of an artificial gene for the synthesis of a replicatable RNA Patent #: 5112734 InventorsAssigneeApplicationNo. 015249 filed on 02/05/1993US Classes:435/6, Involving nucleic acid435/91.1Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.)ExaminersPrimary: Zitomer, Stephanie W.Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C12P 019/34 AbstractNew methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.Other References
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