Bio-analytical separation method
December 27, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesChemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer Fluoroimmunoassaying Non-radioactively labeled microspheres and use of same to measure blood flow Sac having a marker linked thereto Vesicles and use thereof in an assay Assay method employing fluorescent cell incorporative dye Viable cell labelling Volumetric determination of a fluid Fluorophores for encapsulation into liposomes Patent #: 4912208 InventorsAssigneeApplicationNo. 486618 filed on 02/28/1990US Classes:600/504, Measuring blood flow in body portion other than heart128/898, Methods435/29, Involving viable micro-organism435/35, Using radioactive material435/39, Quantitative determination435/40.5, Involving fixed or stabilized, nonliving microorganism, cell, or tissue (e.g., processes of staining, stabilizing, dehydrating, etc.; compositions used therefore, etc.)436/63, BIOLOGICAL CELLULAR MATERIAL TESTED436/164, OPTICAL RESULT436/172With fluorescence or luminescenceExaminersPrimary: Sykes, Angela D.Attorney, Agent or FirmForeign Patent References
International ClassA61B 005/027.5AbstractA separation method for use in conjunction with bio-analytical techniques, such as cell screening and blood flow measurement, in which a carrier bearing a bio-affecting compound comprising a reporter moiety is contacted with an organic extractant which is immiscible with the carrier, so as to produce distinct extractant and carrier phases, with a major amount of the bio-affecting compound originally associated with the carrier being transferred to the extractant phase. The separation method is particularly useful in performing bio-analytical techniques involving fluorometric determinations, as fluorescence efficiency is increased substantially by transfer of a fluorescent reporter from an aqueous carrier phase to the organic extractant phase, and concentration of the fluorescent reporter in the extractant phase prior to fluorescence measurement.Other References
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