Solution sandwich hybridization, capture and detection of amplified nucleic acids
Patent 5374524 Issued on December 20, 1994. Estimated Expiration Date: 
April 27, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
April 27, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesInventorAssigneeApplicationNo. 053842 filed on 04/27/1993US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/810, PACKAGED DEVICE OR KIT436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/22.1, N-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.)536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Parr, MargaretAssistant: Marschel, Ardin H. Foreign Patent References
International ClassesC12Q 001/68C12P 019/34 AbstractA nucleic acid probe assay which combines nucleic acid amplification with solution hybridization using capture and reporter probes followed by or simultaneously with immobilization on a solid support is provided. An assay sensitivity equal to 5 copies of HIV I DNA was achieved.Other References
Field of SearchInvolving nucleic acidPACKAGED DEVICE OR KIT Polynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Acellular exponential or geometric amplification (e.g., PCR, etc.) BIOSPECIFIC LIGAND BINDING ASSAY N-glycosides wherein the N is part of an N-hetero ring which hetero ring is part of a polycyclo ring system containing an N-hetero ring and an additional hetero ring (e.g., rebeccamycin, etc.) DNA or RNA fragments or modified forms thereof (e.g., genes, etc.) Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) Non-coding sequences having no known regulatory function which are adaptors or linkers for vector or gene contruction Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA Probes for detection of animal nucleotide sequences Probes for detection of microbial nucleotide sequences Primers |
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