Method for releasing RNA and DNA from cells
Patent 5374522 Issued on December 20, 1994. Estimated Expiration Date: 
December 20, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 20, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 2473453 3172546 3558066 Method of extracting useful components from microbial cells Test kit for the genetic detection of microorganisms Apparatus for breaking bacterial cells Apparatus for continuously disintegrating cells of microorganisms Patent #: 4476225 InventorsApplicationNo. 711114 filed on 06/21/1991US Classes:435/6, Involving nucleic acid241/2, With cell rupturing or liberation of contained liquids536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/25.4Separation or purification of polynucleotides or oligonucleotidesExaminersPrimary: Fleisher, MindyAttorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C07H 021/02 A47J 019/06 AbstractA method is disclosed for disrupting cells, including microorganisms, and facilitating thereby the release of cellular components including RNA and DNA into solution. Solutions or suspensions of cells are placed in a container with minute beads of various composition. The container is then placed in an ultrasound bath or otherwise subjected to sonication until the cells disrupt releasing their cellular components, including RNA and DNA. The released RNA and DNA are then available for hybridization with genetic probes.Other References
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