Method and apparatus for quantitative fluorescence measurements
September 30, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3604927 Cytological assay procedure Apparatus for eliminating background interference in fluorescence measurements Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples Remote multi-position information gathering system and method Grain damage analyzer Method and apparatus for the measurement of low-level laser-induced fluorescence Fluorescence imaging system Differential binding of membrane potential sensitive materials to lymphocytes Fluorometer InventorsApplicationNo. 953898 filed on 09/30/1992US Classes:356/317, By light250/461.2, Biological cell identification356/417Included with sample excitationExaminersPrimary: McGraw, Vincent P.Assistant: Hantis, K. P. Attorney, Agent or FirmForeign Patent References
International ClassesG01J 003/30G01N 021/64 AbstractMeasurement of the fluorescence of a layer of cells, disposed in a well, with supernatant liquid thereabove, is greatly enhanced in sensitivity by illuminating the cell layer with a beam of light incident thereupon at a first angle and detecting fluorescence emitted by the cells with a detector which views the illuminated cells at a second angle, wherein at least one of the first or second angles is oblique to the cell layer. By so controlling the geometry of the system, the contribution to background fluorescence by the supernatant liquid is greatly minimized. Sensitivity of the technique is further enhanced by restricting the portion of the illuminated cell layer which is viewed by the detector. The technique may be applied to an apparatus for rapidly scanning the fluorescence of a plurality of samples in a multiple well plate.Other References
Field of SearchBy lightMonochromatic (e.g., laser) Included with sample excitation SAMPLE, SPECIMEN, OR STANDARD HOLDER OR SUPPORT (E.G., PLATES OR SLIDES) With significant sample holder or supply BY ELECTROPHORESIS LUMINOPHOR IRRADIATION Methods With ultraviolet source Biological cell identification OPTICAL SENSING APPARATUS Staphylococcus | |
