Recombinant thermostable DNA polymerase from archaebacteria
Patent 5352778 Issued on October 4, 1994. Estimated Expiration Date: December 15, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
536/23.2, Encodes an enzyme435/6, Involving nucleic acid435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/194, Transferring phosphorus containing group (e.g., kineases, etc.(2.7))435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/252.33, Escherichia (e.g., E. coli, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.7, Encodes a microbial polypeptide536/24.32Probes for detection of microbial nucleotide sequences
Recombinant DNA polymeraset from archaebacteria as well as isolated DNA coding for such polymeraset are provided. The isolated DNA is obtained by use of DNA or antibody probet prepared from the DNA encoding T. litoralis DNA polymerate and the T. litoralis DNA polymerate respectively. Also provided are meshocs for producing recombinant archaebacteria thermostable DNA polymerase and methods for enhancing the expression of such polymeraset by identifying, locating and removing introns from within the DNA coding for such DNA polymerases.
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