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Biological insect control agents and methods of use

Patent 5352451 Issued on October 4, 1994. Estimated Expiration Date: Icon_subject October 4, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Assignee

Application

No. 656179 filed on 02/28/1991

US Classes:

424/93.2, Genetically modified micro-organism, cell, or virus (e.g., transformed, fused, hybrid, etc.)435/235.1, VIRUS OR BACTERIOPHAGE, EXCEPT FOR VIRAL VECTOR OR BACTERIOPHAGE VECTOR; COMPOSITION THEREOF; PREPARATION OR PURIFICATION THEREOF; PRODUCTION OF VIRAL SUBUNITS; MEDIA FOR PROPAGATING435/456The polynucleotide is encapsidated within a virus or viral coat

Examiners

Primary: Schwartz, Richard A.
Assistant: Mosher, Mary E.

Attorney, Agent or Firm

International Classes

A01N 063/00
C12N 007/01
C12N 015/00

Abstract

Insect control agents comprising genes encoding proteins affecting the growth, development or behavior of an insect are provided. The gene is either activated to prevent insect molting and pupation or is inactivated to reduce the feeding behavior, inhibit growth and result in the earlier death of the insect host. Such an insect control agent is exemplified by a baculovirus in which the gene encoding ecdysteriod glucosyltransferase has been inactivated. Additionally, such baculoviruses may be further modified to express a protein which affects ecdysis. Methods for producing the insect control agent and methods of controlling insects by exposing them to the insect control agent are also included.

Other References

  • O'Reilly, D. R. et al. 1992 Insect Biochen. Molec. Biol. vol. 22 pp. 313-320
  • Smith, G. E. et al. 1982. Virology vol. 123 pp. 393-406
  • Dougherty et al. (1987) Physiol. Entomol. 12:23-30
  • O'Reilly, D. R. & Miller, L. K. (1990) "Regulation of Expression of a Baculovirus Ecdysteroid UDPglucosyltransferase Gene" J. Virol. 64(3):1321-1328
  • O'Reilly & Miller (1989) "A Baculovirus Blocks Insect Molting by Producing Ecdysteroid UDP-glucosyltransferase" Science 245:1110-1112
  • Lee & Miller (1978) "Isolation of Genotypic Variants of Autographa californica Nuclear Polyhedrosis Virus" J. Virol. 27(3):754-767
  • Kumar & Miller (1987) "Effects of Serial Passage of Autographa californica Nuclear Polyhedrosis Virus in Cell Culture" Virus Res. 7:335-349
  • Hammock, B. D. et al. (1990) "Expression and Effects of the Juvenile Hormone Esterase in a Baculovirus Vector" Nature 344:458-461
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  • Rankin, C. et al. (1988) "Eight Base Pairs Encompassing the Transcriptional Start Point Are the Major Determinant for Baculovirus Polyhedrin Gene Expression" Gene 70:39-49
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  • Koolman and Karlson (1985) "Regulation of Ecdysteroid Titer: Degradation" in Comprehensive Insect Physiology and Biochemistry and Pharmacology, Kercut and Gilbert (eds.), vol. 7, pp. 343-361
  • Miller, L. K. et al. (1983) "Bacterial, Viral, and Fungal Insecticides" Science 219:715-721
  • Kirschbaum, J. B. (1985) "Potential Implication of Genetic Engineering and Other Biotechnologies to Insect Control" Ann. Rev. Entomol. 30:51-70
  • Miller, L. K. (1987) "Expression of Foreign Genes in Insect Cells" in Biotechnology In Invertebrate Pathology and Cell Culture, Maramorosch, K. (ed.), pp. 295-303
  • Miller and Dawes (1979) "Physical Map of the DNA Genome of Autographa californica Nuclear Polyhedrosis Virus" J. Virol. 29:1044-105
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